| 异戊烯基转移酶基因导入番茄及转基因植株再生 |
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| 引用本文： | 张赛群,叶志彪,吴昌银,张启发.异戊烯基转移酶基因导入番茄及转基因植株再生[J].园艺学报,1999,26(6):376-379. |
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| 作者姓名： | 张赛群 叶志彪 吴昌银 张启发 |
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| 作者单位： | 华中农业大学作物遗传改良国家重点实验室,武汉,430070 |
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| 摘 要： | 通过根癌农杆菌（Ａgrobacteriens）介导，利用特异启动子Ｐsag12与异戊烯基转移酶（Ｉsopenyl transferase，ipt）基因构建的嵌合基因Ｐsag12－ipt对５个番茄品种进行遗传转化，结果共获得来自３１个不同外植体的４４株转化再生植株。感染子叶的分化再生及转化植株田间生长正常。对其中部分转化再生植株进行ＰＣＲ检测证明为转基因植株，并且在田间表现出生长旺盛及抗叶片衰老的特性。
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| 关 键 词： | 番茄 异戊烯基转移酶 遗传转化 基因导入 |
| 修稿时间： | 1999-02-01 |
Genetic Transformation of Isopentenyl Transferase Gene to Tomato and Regeneration of Transgenic Plants |
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| Zhang Saiqun,Ye Zhibiao,Wu Changyin,Zhang Qifa.Genetic Transformation of Isopentenyl Transferase Gene to Tomato and Regeneration of Transgenic Plants[J].Acta Horticulturae Sinica,1999,26(6):376-379. |
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| Authors: | Zhang Saiqun Ye Zhibiao Wu Changyin Zhang Qifa |
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| Abstract: | The strain AGS 1 of Agrobacterium tumerfaciens harboring a plasmid carrying an ipt gene driven by the promoter of SAG12 was used to transform tomato.Cotyledons of five tomato cultivars were used and before inoculation,some explants were precultured and some were not.From 31 explants of 5 cultivars 44 transformants have been obtained in this experiment.By PCR analysis on part of the putative transformants,we identified they are transgenic.The transgenic plants showed characteristics of delaying leaf senescence. |
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| Keywords: | Tomato Isopentenyl transferase Genetic transformation |
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