| 基于EST 数据库的葡萄成花途径的预测及分析 |
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| 引用本文： | 刘 丹,慕 茜,李晓鹏,刘更森,李 玉,张彦苹,房经贵.基于EST 数据库的葡萄成花途径的预测及分析[J].园艺学报,2014,41(1):26-34. |
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| 作者姓名： | 刘 丹 慕 茜 李晓鹏 刘更森 李 玉 张彦苹 房经贵 |
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| 作者单位： | 1 南京农业大学园艺学院，南京 210095；2 青岛农业大学园艺学院，青岛 266109 |
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| 基金项目： | 国家自然科学基金项目（31372027） |
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| 摘 要： | 以拟南芥成花途径相关基因序列为探针，对葡萄EST 数据库进行同源检索筛选，获得相应
的同源EST 序列，并以葡萄不同发育时期的不同器官cDNA 为模板，通过半定量RT-PCR 反应研究了葡
萄成花途径相关基因的存在与表达情况，对葡萄成花途径的进行了分析与判断。研究发现，葡萄成花途
径中的基因在不同器官中的不同时期的时空表达存在强弱的差异。
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| 关 键 词： | 葡萄 EST 成花途径 基因 |
Forecasting Analysis of the Grape Flowering Pathway Based on the Grape EST Database |
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| LIU Dan,MU Qian,LI Xiao-peng,LIU Geng-sen,LI Yu,ZHANG Yan-ping,FANG Jing-gui,.Forecasting Analysis of the Grape Flowering Pathway Based on the Grape EST Database[J].Acta Horticulturae Sinica,2014,41(1):26-34. |
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| Authors: | LIU Dan MU Qian LI Xiao-peng LIU Geng-sen LI Yu ZHANG Yan-ping FANG Jing-gui |
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| Institution: | 1College of Horticulture，Nanjing Agricultural University，Nanjing 210095，China；2College of Landscape and Horticulture，Qingdao Agricultural University，Qingdao，Shandong 266109，China |
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| Abstract: | Homologous grape EST sequences were searched against the grape EST database using the
corresponding flowering pathway gene sequences from Arabidopsis as the probes. The existence and
expression of related genes involved in the flowering pathway in grape were further studied by
half-qRT-PCR using cDNA，prepared from different grape organs at various developing stages，as PCR
template，bashed on which，the grape flowering pathways were thoroughly forecasted. The study results
also indicated that the expression levels of the grape flowering pathway genes showed some
spatiotemporal differences. |
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| Keywords: | grape EST flowering pathway genes |
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