| 白菜抗霜霉病基因的RAPD标记 |
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| 引用本文： | 冷月强,侯喜林,史公军.白菜抗霜霉病基因的RAPD标记[J].园艺学报,2007,34(3):763-766. |
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| 作者姓名： | 冷月强 侯喜林 史公军 |
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| 作者单位： | (南京农业大学作物遗传与种质创新国家重点实验室, 南京210095) |
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| 基金项目： | 教育部高等学校博士学科点专项科研基金;国家高技术研究发展计划(863计划) |
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| 摘 要： | 研究了与抗霜霉病基因紧密连锁的RAPD分子标记, 利用白菜抗病品种014和感病品种010杂交的F2群体144个单株为材料, 通过人工接种鉴定, 采用高抗单株和高感单株分别构建抗、感病池, 利用BSA法筛选了560条RAPD引物, 其中引物AY12在抗感病池中扩增出多态性片段AY121238 , 通过F2单株验证后证明AY121238与抗霜霉病基因紧密连锁, 其遗传距离为6.7 cM。
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| 关 键 词： | 白菜 霜霉病 RAPD标记 遗传距离 |
| 文章编号： | 0513-353X（2007）03-0763-04 |
| 修稿时间： | 2006-11-082007-02-28 |
The RAPD Markers Linked to Downy Mildew Resistant Gene in Non-heading Chinese Cabbage (Brassica campestris ssp.chinensis Makino) |
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| LENG Yue-qiang,HOU Xi-lin,SHI Gong-jun.The RAPD Markers Linked to Downy Mildew Resistant Gene in Non-heading Chinese Cabbage (Brassica campestris ssp.chinensis Makino)[J].Acta Horticulturae Sinica,2007,34(3):763-766. |
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| Authors: | LENG Yue-qiang HOU Xi-lin SHI Gong-jun |
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| Institution: | (State Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China) |
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| Abstract: | The objective of this study was to identify RAPD(Random Amplified Polymorphic DNA) markers for downy mildew resistance in non-heading Chinese cabbage.RAPD-PCR(Polymerase Chain Reaction) analysis was conducted using BSA(Bulked Segregant Analysis) of a segregating F2 population which has 144 individuals from a cross between 010(susceptible) and 014(resistant).After performing RAPD-PCR analysis with 560 arbitrary 10-mer primers and agarose-gel electrophoresis,only one RAPD marker AY121238 was identified to be linked to the resistant gene and was determined to be 6.7 cM from the resistant gene. |
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| Keywords: | Non-heading Chinese cabbage Downy mildew RAPD marker Genetic distance |
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