利用SSR 标记进行枣树子代苗父本鉴定

 利用SSR标记技术对‘冬枣’自然授粉实生苗1 728株和‘灵宝大枣’自然授粉实生苗530株进行父本鉴定，利用可能的父本材料从376对引物中筛选出8对条带清晰、多态性较高且具有高度区分度的引物。采用荧光M13毛细管电泳技术，用这8对引物在亲本材料中共检测到45个多态等位条带，每对引物的平均多态条带数为5.625，PIC值变幅为0.58 ~ 0.86，平均为0.71。利用Cervus3.0软件对具有8对引物数据的子代进行父本分析，鉴定出‘冬枣’ב木枣’实生苗399株、‘冬枣’ב小芽枣’实生苗390株、‘冬枣’ב映山红’实生苗357株、‘灵宝大枣’ב紫圆枣’（‘紫枣’）实生苗204株、‘灵宝大枣’ב柿饼枣’实生苗126株。

Application of SSR Markers for the Identification of Paternal Parent for the Seedlings of Chinese Jujube

The paternalparents of 1 728 open-pollinated seedlings of‘Dongzao’（Ziziphus jujuba Mill.）and 530 open-pollinated seedlings of‘Lingbao Dazao’were identified with SSR technique. Eight primer pairs were selected from 376 primer pairs，which amplified distinct，polymorphic segments and showed high differential rate. Twelve candidate parents were analyzed by capillary electrophoresis using fluorescent M13 multi-state detection method. Totally，45 polymorphic alleles were revealed，with an average of 5.625 for each primer pairs. Polymorphism information content values for the primer pairs ranged from 0.58 to 0.86，with an average of 0.71. Using software Cervus3.0，399 hybrids from‘Dongzao’בMuzao’were identified，and 390 from‘Dongzao’בXiaoyazao’，357 from‘Dongzao’בYingshanhong’，204 from ‘Lingbao Dazao’בZiyuanzao（Zizao）’，126 from‘Lingbao Dazao’בShibingzao’were also identified.