| 36 份枣品种SSR 指纹图谱的构建 |
| |
| 引用本文： | 麻丽颖,孔德仓,刘华波,王斯琪,李颖岳,庞晓明.36 份枣品种SSR 指纹图谱的构建[J].园艺学报,2012,39(4):647-654. |
| |
| 作者姓名： | 麻丽颖 孔德仓 刘华波 王斯琪 李颖岳 庞晓明 |
| |
| 作者单位： | （1 北京林业大学计算生物学中心，林木育种国家工程实验室，林木、花卉遗传育种教育部重点实验室，北京 100083；2 河北省沧县枣树国家良种基地，河北沧州 061000） |
| |
| 基金项目： | 中央高校基本业务费项目，林业公益性行业专项 |
| |
| 摘 要： | 利用12 对SSR 引物对36 个枣品种进行分析，采用荧光M13 毛细管电泳技术进行多态检测，共检测到99 个多态位点，每对引物的多态位点数达到8.25，PIC 值变幅为0.62 ~ 0.85，平均为0.75。依据12 对SSR 引物在36 个品种中扩增的特异带型组合，采用引物-带型组合法构建了36 个枣品种的指纹图谱，并对这36 份枣品种做聚类分析，遗传相似系数在0.6667 ~ 0.9444 之间。研究结果为枣树分类、种质鉴定和分子育种提供了重要的工具。
|
| 关 键 词： | 枣 SSR 指纹图谱 毛细管电泳 遗传相似性 |
Construction of SSR Fingerprint on 36 Chinese Jujube Cultivars |
| |
| MA Li-ying,KONG De-cang,LIU Hua-bo,WANG Si-qi,LI Ying-yue,and PANG Xiao-ming.Construction of SSR Fingerprint on 36 Chinese Jujube Cultivars[J].Acta Horticulturae Sinica,2012,39(4):647-654. |
| |
| Authors: | MA Li-ying KONG De-cang LIU Hua-bo WANG Si-qi LI Ying-yue and PANG Xiao-ming |
| |
| Institution: | （1Center for Computational Biology，National Engineering Laboratory for Tree Breeding，Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants，Ministry of Education，Beijing Forestry University，Beijing 100083， China；2National Improved Cultivar Station of Jujube，Cangzhou，Hebei 061000，China） |
| |
| Abstract: | In this study,36 Chinese jujube cultivars were analyzed by capillary electrophoresis using fluorescent M13 multi-state detection method with 12 pairs of newly developed SSR primers. Totally,99 polymorphic alleles were revealed,with an average of 8.25 for each primer pairs. Polymorphism information content values for the primer pairs ranged from 0.62–0.85,with an average of 0.75. A strategy of combining primer pair with distinct alleles for fingerprint construction was developed and applied to the 36 cultivars. The genetic similarities among cultivars range from 0.6667 to 0.9444,on which a phenetic tree showing the relationship among the cultivars was constructed. The present results provide valuable tools for the cultivar classification,germplasm identification and molecular breeding of Chinese jujube. |
| |
| Keywords: | jujube SSR fingerprint capillary electrophoresis genetic similarity |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
