| GMPase超表达对番茄植株抗坏血酸含量及耐冷性相关生理指标的影响 |
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| 引用本文： | 李超汉,张琳,史庆华,李青竹,郭晓青,李霞,于贤昌.GMPase超表达对番茄植株抗坏血酸含量及耐冷性相关生理指标的影响[J].园艺学报,2011,38(4):692-700. |
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| 作者姓名： | 李超汉 张琳 史庆华 李青竹 郭晓青 李霞 于贤昌 |
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| 作者单位： | 1. 山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东,泰安,271018
2. 中国农业科学院蔬菜花卉研究所,北京,100081 |
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| 基金项目： | 国家重点基础研究发展计划项目，国家自然科学基金项目，现代农业产业技术体系建设专项项目，农业部园艺作物遗传改良重点开放实验室资助项目 |
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| 摘 要： | 采用农杆菌介导法获得了GDP–D–甘露糖焦磷酸化酶基因（GMPase）超表达的转基因番茄植株，并且采用Western blot检测到有两株GMPase蛋白表达显著增加。比较了这两个株系和野生型植株中抗坏血酸含量及耐低温能力。与野生型番茄相比，GMPase超表达在常温及低温胁迫下均使GMPase活性提高，使AsA和DHA含量显著增加，降低了低温胁迫下膜脂过氧化产物MDA的积累。
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| 关 键 词： | 番茄 抗坏血酸 GDP–D–甘露糖焦磷酸化酶 转基因 低温胁迫 |
Effects of GMPase Overexpression on Ascorbic Acid Content and Relative Index to Low-temperature Tolerance in Tomato Plants |
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| LI Chao-han,ZHANG Lin,SHI Qing-hua,LI Qing-zhu,GUO Xiao-qing,LI Xia,YU Xian-chang.Effects of GMPase Overexpression on Ascorbic Acid Content and Relative Index to Low-temperature Tolerance in Tomato Plants[J].Acta Horticulturae Sinica,2011,38(4):692-700. |
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| Authors: | LI Chao-han ZHANG Lin SHI Qing-hua LI Qing-zhu GUO Xiao-qing LI Xia YU Xian-chang |
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| Institution: | LI Chao-han1,ZHANG Lin1,SHI Qing-hua1,LI Qing-zhu1,GUO Xiao-qing1,LI Xia1,and YU Xian-chang2,(1State Key Laboratory of Crop Biology,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai'an,Shandong 271018,China,2Institute of Vegetables and Flowers,Chinese Academy of Agriculture Sciences,Beijing 100081,China) |
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| Abstract: | Two transgenic tomato plants of overexpressing GDP-D-mannose pyrophosphorylase gene (GMPase)were obtained by Agrobacterium-mediated transformation,and Western blot analysis indicated that the GMPase protein in transgenic plants was significantly increased.T0 make sure the function of GMPase overexpression,ascorbate content and cold tolerance capacity were studied.Compared with wild-type plants,the GMPase activity and contents of AsA and DHA in both normal and low-temperature conditions were increased and th... |
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| Keywords: | tomato ascorbic acid GDP-D-mannose pyrophosphorylase transgenic low-temperature stress |
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