Alterations in gene expression of sarcoplasmic reticulum Ca2+-ATPase and phospholamban detected by RNA array in spontaneously hypertensive rats |
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Authors: | CHEN Nai-yun HU Shen-jiang DONG Hai-tao |
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Institution: | 1. Department of Cardiology, First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310003, China;
2. Agriculture and Biotechnology Academy, Zhejiang University, Hangzhou 310029, China |
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Abstract: | AIM: To explore the relationship between the alteration in gene expression of sarcoplasmic reticulum Ca2+-ATPase (SERCA) and phospholamban (PLB) in spontaneously hypertensive rats (SHR). METHODS: 294 samples of total RNA were obtained from the tissue of ventriculum , aortic smooth muscle, liver and kidney in SHR and normotensive rats (WKY). RNA array was used to determine the mRNA levels of SERCA and PLB. RESULTS: Compared with age-matched WKY rats, the systolic blood pressure increased higher in 6-week-old SHR (P<0.01). The cardiosomatic ratio was significantly higher in 10-week-old SHR (P<0.01), in cardiac sarcoplasmic reticulum, the mRNA levels of SERCA were significantly increased from 4 weeks (P<0.05 or P<0.01). In the aortic sarcoplasmic reticulum, the mRNA levels of SERCA were significantly increased from 4 weeks to 12 weeks (P<0.05 or P<0.01). There was no significant change in the expression of PLB between the two groups. The ratio of cardiac SERCA and PLB was significantly increased since 6-week-old (P<0.05 or P<0.05)in SHR. The ratio of aortic SERCA and PLB in SHR was significantly increased since 4-week-old (P<0.05 or P<0.01) vs WKY. CONCLUSION: Our results provided the evidence that the abnormalities of intracellular Ca2+ hemostasis in SHR represent the progressive nature of essential hypertension. |
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Keywords: | Hypertension Rats Ca2+-transporting ATPase Gene expression |
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