| 蝴蝶兰褐变外植体的显微结构观察以及褐变成分的初步分析 |
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| 引用本文： | 许传俊,李玲,李红,张铭光.蝴蝶兰褐变外植体的显微结构观察以及褐变成分的初步分析[J].园艺学报,2005,32(6):1111-1116. |
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| 作者姓名： | 许传俊 李玲 李红 张铭光 |
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| 作者单位： | (华南师范大学生命科学学院, 广东省植物发育生物工程重点实验室, 广州510631; 福建省亚热带植物研究所, 厦门361006) |
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| 基金项目： | 广东省自然科学基金项目(003062)，广东省科技计划项目(2005B20901019) |
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| 摘 要： | 对蝴蝶兰褐变外植体进行扫描电镜和显微结构观察, 发现褐变叶片外植体的上表皮已经变形,维管束完全破坏, 被有毒的鞣质填充, 有些薄壁细胞中发现有不明物质沉积。初步分析褐变外植体分泌到培养基中的成分有缩合鞣质。外植体总酚含量和PAL活性随褐变程度加重逐渐增加, 两者呈现显著正相关。
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| 关 键 词： | 蝴蝶兰 褐变 外植体 显微结构 鞣质 |
| 文章编号： | 0513-353X（2005）06-1111-03 |
| 收稿时间： | 2004-12-10 |
| 修稿时间： | 2004-12-102005-01-20 |
Preliminary Studies on the Elements of Browning and the Changes in Cellular Texture of Leaf Explant Browning in Phalaenopsis |
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| Xu Chuanjun,Li Ling,Li Hong,Zhang Mingguang.Preliminary Studies on the Elements of Browning and the Changes in Cellular Texture of Leaf Explant Browning in Phalaenopsis[J].Acta Horticulturae Sinica,2005,32(6):1111-1116. |
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| Authors: | Xu Chuanjun Li Ling Li Hong Zhang Mingguang |
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| Institution: | Cancer Institute, The Second Affiliated Hospital, Medical School of Zhejiang University, Hangzhou 310009, China |
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| Abstract: | The microstructure of Phalaenopsis sp.was examined with microscope and scanning electron microscope.The results showed that the vascular bundle of browning cell filled with tannin and the upper epidermis shriveled;There are something deposited in parenchyma cell.The colorimetric assay demonstrated that the substance extracted from medium was condensed tannins.With the browning of explant,the content of total phenol compounds and the activity of PAL were increased,and the co-relationship analysis showed that they were positive correlation significant at 1% level. |
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| Keywords: | Phalaenopsis Browning Explant Microstructure Tannin |
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