| 番茄抗晚疫病基因Ph-3的RAPD标记 |
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| 引用本文： | 邱夷鹏,李海涛,张子君,邹庆道.番茄抗晚疫病基因Ph-3的RAPD标记[J].园艺学报,2009,36(8):1227-1232. |
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| 作者姓名： | 邱夷鹏 李海涛 张子君 邹庆道 |
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| 作者单位： | ( 沈阳农业大学园艺学院, 沈阳110161; 辽宁省农业科学院蔬菜研究所, 沈阳110161) |
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| 基金项目： | 国家留学基金委(CSC)资助项目，国家科技支撑计划，国家公益性行业(农业)科研专项基金，辽宁省自然科学基金 |
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| 摘 要： | 以番茄抗晚疫病材料CLN2037和感病材料T2-03杂交的F2分离群体的147个单株为试材, 通过抗病性鉴定, 选择高抗单株和高感单株分别建抗、感病池。采用BSA法筛选了230条RAPD引物, 其中引物CCPB272-03 (序列为GGTCGATCTG) 在抗、感病池扩增出多态性片段CCPB272-03740 , 通过F2单株验证后证明CCPB272-03740与抗晚疫病基因Ph-3紧密连锁, 遗传距离为518 cM。
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| 关 键 词： | 番茄 晚疫病 RAPD标记 Ph-3基因 |
| 收稿时间： | 2009-4-15 |
| 修稿时间： | 2009-6-26 |
RAPD Marker of the Resistant Gene Ph-3 for Tomato Late Blight |
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| QIU Yi-peng,LI Hai-tao,ZHANG Zi-jun,ZOU Qing-dao.RAPD Marker of the Resistant Gene Ph-3 for Tomato Late Blight[J].Acta Horticulturae Sinica,2009,36(8):1227-1232. |
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| Authors: | QIU Yi-peng LI Hai-tao ZHANG Zi-jun ZOU Qing-dao |
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| Institution: | ( Horticulture College, Shenyang Agricultural University, Shenyang 110161, China; Vegetable Research Institute, LiaoningAcademy of Agricultural Sciences Shenyang 110161, China) |
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| Abstract: | The objective of this studywas to identify RAPD marker for resistance to late blight in tomato.BSA (Bulked SegregantAnalysis) was used to search for RAPD markers linked to late blight resistant gene Ph-3, using F2 segregating population (147 individuals) derived from a cross of CLN2037 ( resistant) andT2-03 ( susceptible ). Two hundred and thirty decamer primers with arbitrary sequences are chosen for polymerase chain reaction amplication. One RAPD marker CCPB272-03740 ( the sequence of the primer is GGTCGATCTG) was tightly linked to the resistant gene Ph-3 and it was 518 cM from the resistant gene |
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| Keywords: | tomato late blight RAPD marker Ph-3 gene Ph-3 |
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