Impact of ubiquitous inhibitors on the GUS gene reporter system: evidence from the model plants Arabidopsis, tobacco and rice and correction methods for quantitative assays of transgenic and endogenous GUS |
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| Authors: | Simone Fior Paolo D Gerola |
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| Institution: | (1) Department of Structural and Functional Biology, University of Insubria, Via H. Dunant 3, 21100 Varese, Italy |
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| Abstract: | Background The β-glucuronidase (GUS) gene reporter system is one of the most effective and employed techniques in the study of gene regulation
in plant molecular biology. Improving protocols for GUS assays have rendered the original method described by Jefferson amenable
to various requirements and conditions, but the serious limitation caused by inhibitors of the enzyme activity in plant tissues
has thus far been underestimated. |
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