| 青花菜总硫代葡萄糖苷提取工艺的优化 |
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| 引用本文： | 贺霞,张子轩,张宇光,张文泽,白瑞,张静.青花菜总硫代葡萄糖苷提取工艺的优化[J].中国瓜菜,2021(4):62-67. |
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| 作者姓名： | 贺霞 张子轩 张宇光 张文泽 白瑞 张静 |
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| 作者单位： | 山西农业大学园艺学院;山西省设施蔬菜提质增效协同创新中心 |
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| 基金项目： | 山西省应用基础研究项目(201701D221188);山西农业大学引进人才博士科研启动费项目(2013YJ47)。 |
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| 摘 要： | 以青花菜中青9号品种为试材,从灭酶处理、干燥处理和提取条件3个方面对青花菜花球中总硫代葡萄糖苷提取工艺进行优化。运用氯化钯分光光度法,比较分析经过不同处理后的青花菜花球总硫代葡萄糖苷含量,以期筛选出总硫苷提取的最佳工艺。结果表明,不同处理对青花菜花球中总硫代葡萄糖苷含量的影响有一定差异。加入100℃蒸馏水后80℃热处理15 min的灭酶处理,70℃鼓风干燥,乙醇浓度90%、料液比1∶9、提取温度60℃、提取时间30 min的提取条件下测得青花菜花球中总硫苷含量(b)最高,为414.98μmol·g-1,此处理为青花菜花球中总硫苷提取最佳工艺。
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| 关 键 词： | 青花菜 总硫代葡萄糖苷 提取工艺 优化 |
Optimization of extraction process of total glucosinolates from broccoli |
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| HE Xia,ZHANG Zixuan,ZHANG Yuguang,ZHANG Wenze,BAI Rui,ZHANG Jing.Optimization of extraction process of total glucosinolates from broccoli[J].China Cucurbits And Vegetables,2021(4):62-67. |
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| Authors: | HE Xia ZHANG Zixuan ZHANG Yuguang ZHANG Wenze BAI Rui ZHANG Jing |
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| Institution: | (College of Horticulture,Shanxi Agricultural University,Taigu 030801,Shanxi,China;Collaborative Innovation Center of Improving Quality and Increasing Profits for Protected Vegetables in Shanxi,Taigu 030801,Shanxi,China) |
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| Abstract: | In this study,the extraction process of total glucosinolates from broccoli was optimized from three aspects:enzyme-inactivation,drying and grinding,and extraction condition,and the broccoli variety Zhongqing 9 hao was used as material in this study.In order to screen out the best extraction process of total glucosinolates,the total glucosinalates contents of broccoli with different treatments were compared and analyzed by palladium chloride-spectrophotometer.The results showed that the effects of different treatments on the total glucosinolates contents of brocolli were significantly different.Under the conditions of 100℃distilled water,80℃heat treatment for 15 min,70℃air drying,90%ethanol concentration,1:9 ratio of solid to liquid,60℃extraction temperature and 30 min extraction time,the content of total glucosinolates in broccoli was the highest(414.98μmol·g-1),which was the best extraction process of total glucosinolates from broccoli. |
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| Keywords: | Broccoli Total glucosinolates Extraction process Optimization |
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