粒径对平菇栽培用玉米芯发酵料代谢物的影响

为了解玉米芯粒径对平菇栽培用发酵料中代谢物的影响,采用代谢组学技术分析添加不同粒径玉米芯的发酵料中微生物代谢物及其代谢通路。结果表明,添加小粒径玉米芯(D₅₀=0.5 cm)和大粒径玉米芯(D₅₀=1.5 cm)的发酵料中微生物代谢物差异显著。在正离子(POS)和负离子(NEG)模式下分别筛选得到464种和201种差异代谢物,包括芳香族化合物、氨基酸、糖及醇类、脂质、生物碱等。差异代谢物分别富集到90条(POS模式)和94条(NEG模式)代谢通路,差异显著的有2条,分别为源自鸟氨酸、赖氨酸、烟酸生物合成生物碱和组氨酸代谢。说明不同粒径玉米芯发酵料中微生物代谢物具有显著差异,为发酵料栽培平菇原料选择提供了理论依据。

Analysis of metabolic differences in fermentation materials for Pleurotus ostreatus cultivation adding corncobs with different particle sizes based on the metabolomics

Metabolomics technique was used to analyze the differences of microbial metabolites in fermentation materials for Pleurotus ostreatus cultivation adding corncobs with different particle sizes.Results showed a significant difference between microbial metabolites in fermentation materials adding small particle(D₅₀=0.5 cm)and large particle(D₅₀=1.5 cm)of corncobs.464 and 201 differential metabolites were screened at POS and NEG modes,respectively,including aromatic compounds,amino acids,sugars and alcohols,lipids,alkaloids.The analysis of KEGG metabolic pathway showed that differential metabolites were enriched in 90(POS mode)and 94(NEG mode)metabolic pathways,respectively,and two of which were significantly different.They were Biosynthesis of alkaloids derived from ornithine,lysine and nicotinic acid and Histidine metabolism.The results indicated that there were significant differences between the microbial metabolites in the fermentation materials adding corncobs with different particle sizes,which provided important information for the selection of cultivation materials and the improvement of cultivation techniques of P.ostreatus.