| 基于分子检测技术的番茄和茄子嫁接番茄砧木培育方法 |
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| 引用本文： | 程 琳,于彩云,魏美甜,张海娟,王文涛,毛瑞喜,陈福东,武玉芬,韩宇睿,王 雪,程 斐,许 勇,别之龙.基于分子检测技术的番茄和茄子嫁接番茄砧木培育方法[J].蔬菜,2023(10):19-25. |
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| 作者姓名： | 程 琳 于彩云 魏美甜 张海娟 王文涛 毛瑞喜 陈福东 武玉芬 韩宇睿 王 雪 程 斐 许 勇 别之龙 |
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| 作者单位： | （1.山东寿光蔬菜种业集团有限公司/山东省设施蔬菜分子育种重点实验室/山东省寿光蔬菜产业集团有限公司/农业农村部设施蔬菜种质创新重点实验室/山东省蔬菜工程技术研究中心，山东 寿光 262700；2.淄博市数字农业农村发展中心，山东 淄博 255020；3.山东省种子管理总站，山东 济南 250131；4.青岛农业大学，山东 青岛 266000；5.北京市农林科学院，北京 100097；6.华中农业大学，湖北 武汉 430000） |
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| 基金项目： | 基金项目：2021年度山东省重点研发计划重大科技创新工程项目（2021LZGC017）；2022年度山东省重点研发计划竞争性创新平台项目（2022CXPT011）；2022年度山东省重点研发计划农业良种工程项目（2022LZGCQY003）；2022年度泰山产业领军人才。 |
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| 摘 要： | 摘要：为了获得能够同时作为番茄和茄子嫁接的砧木，选用自主培育的多抗番茄砧木组合26个，国内优良番茄嫁接砧木品种4个和国外引进番茄嫁接砧木3个，分别进行抗病基因包括抗根结线虫的Mi1和Mi-2、抗颈腐根腐的Frl、抗枯萎病的I-2/5和抗黄萎病的Ve1基因的分子标记检测；同时还测定了砧木品种根、茎的主要生理指标，包括壮苗指数（茎粗/株高）、根系质量和下胚轴长度等。结果表明：番茄砧木嫁接试验中，番茄接穗的嫁接成活率和移栽成活率均在95.0%以上，茄子接穗的嫁接成活率和移栽成活率均在85.0%以上。筛选出的HC483、HC484、HC490、HC493、HC494、HC495、500-2、B2和Y1共9个砧木品种综合表现较为突出，而且以这些番茄砧木培育得到的种苗长势旺盛、根系发达且抗多种病虫害。
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| 关 键 词： | 关键词：番茄 砧木 分子检测 嫁接 |
Cultivation Method of Tomato and Eggplant Grafted Rootstocks Based on Molecular Detection Technology |
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| CHENG Lin,YU Caiyun,WEI Meitian,ZHANG Haijuan,WANG Wentao,MAO Ruixi,CHEN Fudong,WU Yufen,HAN Yurui,WANG Xue,CHENG Fei,XU Yong,BIE Zhilong.Cultivation Method of Tomato and Eggplant Grafted Rootstocks Based on Molecular Detection Technology[J].Vegetables,2023(10):19-25. |
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| Authors: | CHENG Lin YU Caiyun WEI Meitian ZHANG Haijuan WANG Wentao MAO Ruixi CHEN Fudong WU Yufen HAN Yurui WANG Xue CHENG Fei XU Yong BIE Zhilong |
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| Institution: | (1. Shandong Shouguang Vegetable Seed Industry Group Co., Ltd./Shandong Province Key Laboratory of Molecular Breeding of Facility Vegetables/Shandong Shouguang Vegetable Industry Group Co., Ltd./Key Laboratory of Facility Vegetable Germplasm Innovation of Ministry of Agriculture and Rural Affairs/Shandong Vegetable Engineering Technology Research Center, Shouguang 262700, China; 2. Zibo Digital Agriculture Rural Development Center, Zibo 255020, China; 3. Shandong Seed Administration Station, Jinan 250131, China; 4. Qingdao Agricultura University, Qingdao 266000, China; 5. Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; 6. Huazhong Agriculture University, Wuhan 430000, China) |
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| Abstract: | Abstract: In order to obtain rootstocks that can be grafted for both tomato and eggplant, 26 combinations of self-cultivated multi-resistance tomato rootstocks, 4 domestic excellent tomato grafted rootstocks and
3 imported tomato grafted rootstocks were selected to detect disease resistance genes respectively, such as molecular marker detection of Mi1 and Mi-2 genes against root-knot nematodes, Frl gene against root rot, I-2/5 gene against wilt and Ve1 gene against verticillium wilt. At the same time, the main physiological indexes of roots and stems were measured, including seedling strength index (stem diameter/plant height), root mass and hypocotyl length. The results showed that the survival rate of tomato scion grafting and transplanting was more than 95.0% and that of eggplant scion grafting and transplanting was more than 85.0% with tomato rootstocks. A total of 9 rootstock cultivars including HC483, HC484, HC490, HC493, HC494, HC495, 500-2, B2 and Y1, showed outstanding comprehensive performance, and the seedlings cultivated from this tomato rootstocks had strong vigor, developed roots and resistance to multiple diseases and pests. |
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| Keywords: | Keywords: tomato rootstock molecular detection grafting |
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