云南变绿红菇的遗传多样性与群体遗传分化研究

以昆明、曲靖、楚雄、玉溪、昭通、临沧、大理等7个变绿红菇地理居群的30个子实体样本为试验材料,基于ITS序列和LSU序列,对其分子变异、遗传多样性、群体遗传分化进行分析。结果:30个样品中,基于ITS序列检测到18个单倍型,基于LSU序列检测到10个单倍型;基于ITS序列和LSU序列分别构建的单倍型系统发育树及30个样品的系统发育树显示,各地理居群的遗传距离与地理距离之间没有形成对应关系,二者无明显相关性。基于两个序列的分析结果均显示,各地理居群内单倍型多样性较丰富,而核苷酸多样性低。表明变绿红菇在不同的地理居群中的适应能力强,遗传多样性较低,绝大部分遗传分化来自于个体间的差异。

Genetic variation and population genetic differentiation of Russula virescens in Yunnan

In order to find out the genetic diversity and differentiation of Russula virescens population distributed in different areas of Yunnan.The fruiting bodies of Russula virescens from 7 geographical populations,namely Kunming(KM),Qujing(QJ),Chuxiong(CX),Yuxi(YX),Zhaotong(ZT),Lincang(LC)And Dali(DL),were used as test materials.Molecular variation,genetic diversity and population genetic differentiation of 30 samples were analyzed based on ITS and LSU sequences.The results showed that 18 haplotypes were detected based on ITS sequence and 10 haplotypes were detected based on LSU sequence.The haplotype phylogenetic tree constructed based on ITS sequence and LSU sequence showed that there was no corresponding relationship between genetic distance and geographical distance in each geographical population,and there was no significant correlation between them.The results of the two sequences analysis showed that the haplotype diversity was rich in each geographic population,while the nucleotide diversity was low,indicating that the genetic diversity of Russula virescens was low;Fst values among different geographical populations were basically less than 0.15,indicating that the degree of genetic differentiation was low,and most of the differentiation came from differences among individuals.