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楔叶茶藨组织培养研究
引用本文:李金英,张志东,李亚东,吴林,宋宏伟.楔叶茶藨组织培养研究[J].北方园艺,2009(6).
作者姓名:李金英  张志东  李亚东  吴林  宋宏伟
作者单位:吉林农业大学,园艺学院,吉林,长春,130118;吉林省农业科学院,吉林,长春,130033
基金项目:吉林省科技厅资助项目(20040701-1);;农业部948重点资助项目(2006-G25);;科技部资助项目(2005DKA21002-25)
摘    要:试验采用楔叶茶藨水培新梢为外植体,进行了组织培养研究,成功的建立了无性繁殖体系,即初代培养基:MS+BA 1.0 mg/L+IBA 0.1 mg/L;增殖培养基:MS+BA 1.0 mg/L+IBA 0.1~0.2 mg/L+GA30.25~0.5 mg/L。

关 键 词:楔叶茶藨  组织培养

Study on Tissue culture of Ribes diacantha Pall
LI Jin-ying,ZHANG Zhi-dong,LI Ya-dong,WU Lin,SONG Hong-wei.Study on Tissue culture of Ribes diacantha Pall[J].Northern Horticulture,2009(6).
Authors:LI Jin-ying  ZHANG Zhi-dong  LI Ya-dong  WU Lin  SONG Hong-wei
Institution:1.College of Horticulture;Jilin Agricultural University;Changchun;Jilin 130118;China;2.Jilin Academy of Agricultural Sciences;Jilin 130033;China
Abstract:The new shoots from water cultured branches of Ribes diacantha Pall were taken as experiment.The optimum media for initiating,proliferating,and rooting cluture were established.The initial culture medium of Ribes diacantha Pall was MS supplemented with BA 1.0 mg/L and IBA 0.1 mg/L;The experiment of bud multiplication showed that:the best sub-culture medium for it in vitro was MS medium containing BA 1.0 mg/L,IBA 0.1~0.2 mg/L and GA30.25~0.5 mg/L.
Keywords:Ribes diacantha Pall  Plant tissue culture  
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