| 紫苏的组织培养及快繁技术研究 |
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| 引用本文： | 黄美娟,张蕾,黄海泉.紫苏的组织培养及快繁技术研究[J].北方园艺,2008(1):198-200. |
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| 作者姓名： | 黄美娟 张蕾 黄海泉 |
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| 作者单位： | 西南林学院,园林学院,云南,昆明,650224 |
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| 基金项目： | 云南省高校园林植物与观赏园艺重点实验室资助项目 |
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| 摘 要： | 以紫苏(Perilla frutescens(Linn)Britt)的种子获得无菌苗,取无菌苗的带节茎段为外植体,对影响紫苏丛芽增殖和生根的主要因子进行了研究.结果表明:紫苏种子较好的灭菌方法为:75%酒精20 s 0.1%升汞8 min;增殖培养基为:MS 6-BA 2.0 mg/L NAA 0.1 mg/L;生根培养基为:1/2 MS 6-BA 1.0 mg/L NAA 0.1 mg/L;且移栽后成活率可达90%以上.
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| 关 键 词： | 紫苏 组织培养 快速繁殖 |
| 文章编号： | 1001-0009（2008）01-0198-03 |
| 收稿时间： | 2007-10-30 |
| 修稿时间： | 2007年10月30 |
Studies on Tissue Culture and Rapid Propagation of Perilla frutescens (Linn) Britt |
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| HUANG Mei-juan,ZHANG Lei,HUANG Hai-quan.Studies on Tissue Culture and Rapid Propagation of Perilla frutescens (Linn) Britt[J].Northern Horticulture,2008(1):198-200. |
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| Authors: | HUANG Mei-juan ZHANG Lei HUANG Hai-quan |
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| Abstract: | The seeds of Perilla frutescens(Linn)Britt were used as explants to germinate and develop into the aseptic plantlets in vitro.The primary factors influenced the proliferation and rooting of Perilla frutescens(Linn)Britt in vitro were studied by using its aseptic stems with nodes as explants.The results indicated that the optimal sterilization method for seeds of Perilla frutescens(Linn)Britt was 75% ethanol 20 s 0.1% HgCl 8 min;that its optimal proliferation culture medium among all tested media was MS 6-BA 2.0 mg/L NAA 0.1 mg/L;that its optimal rooting culture medium among all tested ones was 1/2 MS 6-BA 1.0 mg/L NAA 0.1 mg/L. |
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| Keywords: | Perilla frutescens(Linn)Britt Tissue culture Rapid propagation |
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