| 罗默碱对梨锈病菌抑制机理的初步研究 |
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| 引用本文： | 刘小红,邓业成,唐煌,骆海玉,宁蕾,秦卉.罗默碱对梨锈病菌抑制机理的初步研究[J].北方园艺,2011(12):122-124. |
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| 作者姓名： | 刘小红 邓业成 唐煌 骆海玉 宁蕾 秦卉 |
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| 作者单位： | 广西师范大学生命科学学院,珍稀濒危动植物生态与环境保护省部共建教育部重点实验室,广西,桂林,541004;广西师范大学,药用资源化学与药物分子工程教育部重点实验室,广西,桂林,541004 |
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| 基金项目： | 珍稀濒危动植物生态与环境保护省部共建教育部重点实验室研究基金资助项目(桂科能1001Z014); 广西自然科学基金资助项目(桂科自0991097) |
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| 摘 要： | 以生长速率法测定罗默碱对梨锈病菌菌丝生长的抑制作用,以倍比稀释法测定罗默碱最小抑菌浓度(MIC)和最小杀菌浓度(MFC),以外渗电导法测出罗默碱对梨锈病菌菌丝体细胞膜渗透性的影响,并且测定了罗默碱对菌体可溶性蛋白含量的影响,探讨罗默碱对梨锈病菌的抑制活性及抑制机理。结果表明:罗默碱对梨锈病菌有较强的抑制活性,且浓度越高,抑制作用越强,其MIC为0.25 g/L,MFC为0.5 g/L;用0.2 g/L的罗默碱处理梨锈病菌菌丝体,结果显示其细胞膜的相对渗透率为53.49%,高出对照21.82%;用0.2 g/L的罗默碱处理梨锈病菌菌丝体,其蛋白质含量为2.5 mg/g,对蛋白质合成的抑制率为60.06%。说明罗默碱对梨锈病菌具有较强的抗菌作用;可影响梨锈病菌菌丝体细胞膜的渗透性和可溶性蛋白的含量。
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| 关 键 词： | 罗默碱 梨锈病菌 抑制活性 抑制机理 |
Preliminary Studies on the Antifungal Mechanism of Roemerine Against Gymnosporangium haraeanum Syd. |
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| LIU Xiao-hong,DENG Ye-cheng,TANG Huang,LUO Hai-yu,NIN Lei,QIN Hui.Preliminary Studies on the Antifungal Mechanism of Roemerine Against Gymnosporangium haraeanum Syd.[J].Northern Horticulture,2011(12):122-124. |
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| Authors: | LIU Xiao-hong DENG Ye-cheng TANG Huang LUO Hai-yu NIN Lei QIN Hui |
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| Institution: | LIU Xiao-hong1,DENG Ye-cheng1,TANG Huang2,LUO Hai-yu1,NIN Lei1,QIN Hui1(1.College of Life Science,Guangxi Normal University,Guilin,Guangxi 541004,2.Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources,Ministry of Education of China,Guangxi 541004) |
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| Abstract: | Fungal inhibitory activity of roemerine against G.haraeanum Syd was determined using a growth rate method.And doubling dilution method was used to determined the minimal inhibitory concentration(MIC) and minimal fugicide concentration(MFC),leakage conductivity method using roemerine mycelia of G.haraeanum Syd cell membrane permeability,and determined roemerine on soluble protein content of cell.To explore the antifungal activity and potential mechanism of roemerine against Gymnosporangium haraeanum Syd.The ... |
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| Keywords: | roemerine Gymnosporangium haraeanum Syd inhibition effect inhibition mechanism |
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