| 茶树EST-SSRs信息分析 |
| |
| 引用本文： | 程小毛,王璇,黄晓霞.茶树EST-SSRs信息分析[J].北方园艺,2011(10):108-111. |
| |
| 作者姓名： | 程小毛 王璇 黄晓霞 |
| |
| 作者单位： | 西南林业大学,园林学院,云南,昆明,650224 |
| |
| 基金项目： | “省部级重点学科、省高校重点实验室及校实验室共享平台”资助项目; 西南林业大学校级重点基金资助项目(110909) |
| |
| 摘 要： | 从NCBI公共数据库中公布的茶树表达序列标签中获得12378条茶树EST,通过CAP3软件进行拼接去冗余后再用Bathprimer3 vl.0(http://wheat.pw.usda.gov/demos/BatchPrimer3/)在线软件查找微卫星(Microsatellite,SSR)重复序列。结果表明:通过前处理得到全长为2 307.014kb的无冗余EST4779条,从中检索出27种重复基元类型共207个SSR,分布于156条EST中,出现频率是3.26%。其中包括二核苷酸重复72个(34.78%),三核苷酸重复88个(42.51%),四核苷酸重复31个(14.98%),五核苷酸重复16个(7.73%)。AG/CT和ACC/GGT分别是二、三核苷酸中的优势重复类型,分别占二、三核苷酸重复的86.11%和25.00%。
|
| 关 键 词： | 茶树 EST SSR |
Analysis of SSR Information in EST Resource of Tea Plants |
| |
| CHENG Xiao-mao,WANG Xuan,HUANG Xiao-xia.Analysis of SSR Information in EST Resource of Tea Plants[J].Northern Horticulture,2011(10):108-111. |
| |
| Authors: | CHENG Xiao-mao WANG Xuan HUANG Xiao-xia |
| |
| Institution: | CHENG Xiao-mao,WANG Xuan,HUANG Xiao-xia (College of Landscape Architecture,Southwest Forestry University,Kunming,Yunnan 650224) |
| |
| Abstract: | 12378 ESTs of tea plants in the public database of NCBI were downloaded and some of them redundant were removed using software CAP3,then resulting in 4779 non-redundant ESTs with total length of 2 307.014 kp.Total of 207 SSRs distributed in 156 ESTs were detected,accounting for 3.26%of the non-redurdant ESTs.The distribution distance of EST-SSRs was 1/1.11 kb.The dinucleotide and trinucleotide repeatsis were the main types,accounting for 34.78%and 42.51%of all the SSRs,respectively.AG/CT and ACC/GGT were th... |
| |
| Keywords: | tea plant EST SSR |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
