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培养基优化提高灰树花菌丝体的研究
引用本文:王玉,李政,郝利民,班立桐.培养基优化提高灰树花菌丝体的研究[J].北方园艺,2011(4):190-191.
作者姓名:王玉  李政  郝利民  班立桐
作者单位:天津农学院农学系,天津,300384;天津工业大学纺织学院,天津,300160;总后勤部军需装备研究所,北京,100010
摘    要:为缩短灰树花菌种扩培的时间,缩短灰树花的培养周期,以马铃薯、葡萄糖、蛋白胨、KH2PO4、VB1为因素,设计L16(45)正交实验,对灰树花液体菌种的最优发酵条件进行了研究。结果表明:最适合液体菌种制作的培养基为(g/L):马铃薯200,葡萄糖24,蛋白胨2.5,KH2PO41,VB1 0.005;采用优化后的培养基,灰树花菌丝干重由原始培养基的26 g/L提高到35.4 g/L。

关 键 词:灰树花  培养基优化  菌丝干重

Study on Improving Mycelium of Grifola frondosa by Optimization of Medium
WANG Yu,LI Zheng,HAO Li-min,BAN Li-tong.Study on Improving Mycelium of Grifola frondosa by Optimization of Medium[J].Northern Horticulture,2011(4):190-191.
Authors:WANG Yu  LI Zheng  HAO Li-min  BAN Li-tong
Institution:WANG Yu~1,LI Zheng~2,HAO Li-min~3,BAN Li-tong~1 (1.Department of Agronomy,Tianjin Agricultural University,Tianjin 300384;2.School of Textile and Clothing Polytechnic University,Tianjin 300160;3.Ouartermaster Equipment Institute of General Logistics Department of PLA,Beijing 100010)
Abstract:In order to decrease the period for culture of Grifola frondosa,the optimization of medium for growth of Grifola frondosa were studied.The potato,glucose,peptone,potassium dihydrogen phosphate and VB1 were chosen for designing the orthogonal experiment L16(45) and the optimal medium were(g/L):potato 200,glucose 24,peptone 2.5,KH2PO4 1 and VB1 0.005.Under the medium,the mycelium dry weight of Grifola frondosa increased to 35.4 g/L (26 g/L in initial medium).
Keywords:Grifola frondosa  optimization of fermentation medium  mycelium dry weight
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