| 南昆山莪术的组培快繁技术研究 |
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| 引用本文： | 张施君,刘念,盛爱武,吴国江.南昆山莪术的组培快繁技术研究[J].北方园艺,2011(4):161-163. |
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| 作者姓名： | 张施君 刘念 盛爱武 吴国江 |
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| 作者单位： | 中国科学院华南植物园,广东广州,510650;仲恺农业工程学院园艺与园林学院,广东广州,510225;中国科学院研究生院,北京,100049;仲恺农业工程学院园艺与园林学院,广东广州,510225;中国科学院华南植物园,广东广州,510650 |
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| 基金项目： | 广东省科技攻关资助项目，中科院百人计划资助项目 |
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| 摘 要： | 以南昆山莪术的根状茎腋芽为外植体,采用MS基本培养基,附加不同种类、不同浓度的植物生长调节剂,进行了不定芽诱导、丛生芽继代、试管苗生根等研究,探索其组织培养和离体繁殖的适宜务件。结果表明:在MS+6-BA 5.0 mg/L+NAA 0.1 mg/L培养基中,不定芽的诱导率最高,达到85%;MS+TDZ 0.5 mg/L培养基最有利于丛生芽的增殖,增殖系数达到13.60;试管苗生根以1/2MS+NAA 0.2 mg/L培养基最好;当试管苗长至6 cm时出瓶移栽,成活率可达95%以上。
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| 关 键 词： | 南昆山莪术 组织培养 腋芽 |
Study on Tissue Culture and Rapid Propagation of Curcuma nankunshanensis |
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| ZHANG Shi-jun,LIU Nian,SHENG Ai-wu,WU Guo-jiang.Study on Tissue Culture and Rapid Propagation of Curcuma nankunshanensis[J].Northern Horticulture,2011(4):161-163. |
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| Authors: | ZHANG Shi-jun LIU Nian SHENG Ai-wu WU Guo-jiang |
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| Institution: | ZHANG Shi-jun~(1,2,3),LIU Nian~2,SHENG Ai-wu~2,WU Guo-jiang~1 (1.South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,Guangdong 510650;2.College of Agriculture and Landscape Architecture,Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong 510225;3.College of Graduate University of the Chinese Academy of Sciences,Beijing 100049) |
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| Abstract: | A protocol was outlined for plant rapid propagation using young vegetative bud as explants from rhizomes for Curcuma nankunshanensis,an attractive tropical ornamental.The results showed that the optimal medium for shoot inducing was MS+6-BA 5.0 mg/L+NAA 0.2 mg/L with an inducing rate of 85%.TDZ showed dramatic effects on plantlet propagation,with a mean of 13.60 shoots per explant on MS medium with 0.5 mg/L TDZ.Rooting of the shoots was readily achieved on subsequent 1/2MSmedium + NAA 0.5 mg/L.Complete plantlets(6 cm in length) were transferred to soil in greenhouse for one month and then transplanted in the field.About 95%of the plants survived to maturity. |
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| Keywords: | Curcuma nankunshanensis tissue culture axillary bud |
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