| 西南桦愈伤组织培养试验 |
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| 引用本文： | 陈荣,冯立新,刘颖,杨跃生.西南桦愈伤组织培养试验[J].北方园艺,2011(16):158-160. |
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| 作者姓名： | 陈荣 冯立新 刘颖 杨跃生 |
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| 作者单位： | 华南农业大学生命科学院与药用植物研究中心,广东广州510642;广西生态工程职业技术学院生态工程系,广西柳州545004;广西生态工程职业技术学院生态工程系,广西柳州,545004;华南农业大学生命科学院与药用植物研究中心,广东广州,510642 |
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| 基金项目： | 广西壮族自治区林业厅“十五”科技项目(桂林科字(2001)第80号) |
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| 摘 要： | 以西南桦60 d龄无菌苗茎、根、叶为外植体,研究不同种类和浓度的植物生长调节剂对西南桦愈伤组织诱导的影响.结果表明:当TDZ浓度为0.05 mg/L时诱导效果较好.BA 1.5mg/L+NAA 0.2 mg/L组合愈伤组织鲜重较高,KT 1.5 mg/L+NAA 0.2mg/L的激素组合诱导的愈伤组织质量高,大部分愈伤组织表面疏松,灰白色,并有大量再生根缠绕.叶外植体用于愈伤组织诱导培养比茎、根外植体效果好.生长曲线表明,最佳的继代培养时间为45 d.
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| 关 键 词： | 西南桦 组织培养 生长曲线 |
Experimental Report on Inducement of Callus in Betula alnoides |
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| CHEN Rong
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FENG Li-xin
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LIU Ying
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YANG Yue-sheng.Experimental Report on Inducement of Callus in Betula alnoides[J].Northern Horticulture,2011(16):158-160. |
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| Authors: | CHEN Rong FENG Li-xin LIU Ying YANG Yue-sheng |
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| Institution: | CHEN Rong1,2,FENG Li-xin2,LIU Ying1,YANG Yue-sheng1 (1.Center for Medicinal Plant Research,College of Life Science,South China Agricultural University,Guangzhou,Guangdong 510642,2.Department of Eco-engineering,Guangxi Eco-engineering Vocational and Technical College,Liuzhou,Gansu 545004) |
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| Abstract: | Leaf,root and stem explants were taken from six-months-old in vitro grown plants of Betula alnoides B,Callus inducing was studied under culture media with different kinds and concentrations of hormones.The results indicated that 0.05 mg/L TDZ was suitable for the induction of callus.The highest weight had got with BA 1.5 mg/L+NAA 0.2 mg/L.Calluses were enlarged and rooting with KT 1.5 mg/L+NAA 0.2 mg/L,on this condition the callus grew well and offered the foundation of further study.The leaf explant was be... |
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| Keywords: | Betula alnoides tissue culture callus growth curve |
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