| 分蘖洋葱叶片RNA提取方法的比较和分析 |
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| 引用本文： | 黄钰,李旭双,陈典,王勇.分蘖洋葱叶片RNA提取方法的比较和分析[J].北方园艺,2011(14):111-113. |
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| 作者姓名： | 黄钰 李旭双 陈典 王勇 |
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| 作者单位： | 东北农业大学园艺学院,黑龙江,哈尔滨,150030;东北农业大学园艺学院,黑龙江,哈尔滨,150030;东北农业大学园艺学院,黑龙江,哈尔滨,150030;东北农业大学园艺学院,黑龙江,哈尔滨,150030 |
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| 基金项目： | 黑龙江省高校重点实验室开放基金资助项目(GS2009004); 农业部公益性行业资助项目(200903018) |
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| 摘 要： | 以新鲜的分蘖洋葱叶片为材料,比较了Trizol法、Trizol改进法、SDS法、SDS改进法以及柱式植物RNA提取法提取总RNA的效果。结果表明:Trizol法和Trizol改进法所提取的RNA得率很低,且不能有效除去叶片中的多糖成分;SDS改进法能够有效的去除分蘖洋葱叶片中的多糖,提取的RNA中28S RNA亮度约为18S RNA的2倍,OD260/OD280值介于1.7~2.2之间,但RNA的得率偏低,约为56.32μg/g;柱式植物RNA提取法提取的RNA 28S、18S、5S条带清晰,完整性好,RNA的得率最高,为123.58μg/g,完全适合于分蘖洋葱进一步的分子生物学研究。
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| 关 键 词： | RNA 分蘖洋葱 SDS改进法 柱式植物RNA提取法 |
Comparison of Different Methods for Total RNA Extraction from Leaves of Tillering Onions |
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| HUANG Yu,LI Xu-shuang,CHEN Dian,WANG Yong.Comparison of Different Methods for Total RNA Extraction from Leaves of Tillering Onions[J].Northern Horticulture,2011(14):111-113. |
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| Authors: | HUANG Yu LI Xu-shuang CHEN Dian WANG Yong |
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| Institution: | HUANG Yu,LI Xu-shuang,CHEN Dian,WANG Yong(College of Horticulture,Northeast Agricultural University,Harbin,Heilongjiang 150030) |
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| Abstract: | Fresh leaves of tillers onion were used as material,extracted results of total RNA by the method of trizol,modified trizol,SDS,modified SDS and the TIANDZ column plant RNAout were compared.The results showed that trizol method and modified trizol extracted RNA yield was very low,and can not effectively remove the polysaccharide in the leaves.The modified SDS extraction was capable of efficiently removing polysaccharides,the brightness 28S RNA of the RNAs isolated by the modified SDS was two times as much as... |
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| Keywords: | RNA tillering onions modified SDS TIANDZ column plant RNAout |
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