| 紫阳花组培繁殖技术研究 |
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| 引用本文： | 唐晓杰,胡佰策,程广有.紫阳花组培繁殖技术研究[J].北方园艺,2011(15):180-181. |
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| 作者姓名： | 唐晓杰 胡佰策 程广有 |
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| 作者单位： | 北华大学林学院,吉林吉林,132013;吉林省林业调查规划院,吉林长春,130022 |
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| 摘 要： | 以紫阳花的茎段、茎尖、叶片为外植体,在MS培养基中加入不同质量分数的6-苄基腺嘌呤(6-BA)和吲哚丁酸(IBA)进行增殖培养.结果表明:外植体表面灭菌是一个重要环节,适宜紫阳花外植体表面灭菌方法是:利用0.1％氯化汞(HgCl2)灭菌7min.不同类型的紫阳花外植体在初代培养时增殖效果不同,茎尖在培养过程中,首先伸长生长,然后陆续长出侧芽,表明紫阳花的茎尖是比较适合用来进行增殖培养的外植体.继代培养时以MS+6-BA 1.0 mg/L+IBA0.1 mg/L为紫阳花较适宜的增殖培养基.紫阳花侧芽在1/2MS+IBA 0.2 mg/L培养基中生根率和单苗根数量均较高,说明1/2MS+IBA 0.2 mg/L为较好的不定根诱导培养基.
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| 关 键 词： | 紫阳花 组织培养 繁殖 |
The Technology of Propagation of Hydrangea macrophylla by Tissue Culture |
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| TANG Xiao-jie
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HU Bai-ce
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CHENG Guang-you.The Technology of Propagation of Hydrangea macrophylla by Tissue Culture[J].Northern Horticulture,2011(15):180-181. |
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| Authors: | TANG Xiao-jie HU Bai-ce CHENG Guang-you |
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| Institution: | TANG Xiao-jie1,HU Bai-ce2,CHENG Guang-you1(1.College of Forestry,Beihua University,Jilin,Jilin 132013,2.Institute of Forestry Inventory and Planning of Jilin Province,Changchun,Jilin 130022) |
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| Abstract: | Hydrangea macrophylla propagation were studied for planting stem or leaf in culture medium with different 6-BA or IBA.The results showed that the good method for surface sterilization of explant was soaking them in 0.1% HgCl27 min.Propagation was different because of explant type.Bud stem was good for propagation because it growing first and regenerating lateral bud.The propagation medium of Hydrangea macrophylla was MS+6-BA 1.0 mg/L+IBA 0.1 mg/L.Adventitious root regeneration medium was 1/2MS+IBA 0.2 mg/L. |
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| Keywords: | Hydrangea macrophylla tissue culture propagation |
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