橡胶树死皮病黄色体差异表达蛋白的初步分析

橡胶树"死皮病"给橡胶种植业带来严重的危害.为了更好地了解和阐明死皮病发生、发展的分子机制,研究应用双向凝胶电泳技术(two-dimensional gel electrophshiya/oresis,2-DE)比较橡胶树死皮株与健康株胶乳黄色体蛋白质组表达的差异.采用TCA/丙酮沉淀法提取橡胶树死皮株与健康株黄色体蛋白质并采用固相pH梯度(immobjlized pH graalient,IPG)双向凝胶电泳分离两种材料蛋白质,凝胶经银染显色后,用PDQuest图像分析软件进行比较分析、识别差异表达的蛋白质.成功获得橡胶树死皮株与健康株胶乳黄色体的双向凝胶电泳图谱.鉴定出13个蛋白差异点,其中10个上调表达,3个下调表达.并应用质谱技术鉴定了其中部分表达差异的蛋白质点,对渗调蛋白进行功能分析,认为渗调蛋白在死皮株中表现下调的情形可能与死皮病的发生有一定的关系.

Preliminary Analysis of the Proteins Expressed Differentially of Lutoid on the Latex of Tapping Panel Dryness(TPD) in Rubber Tree (Hevea brasiliensis Muell. Arg.)

Tapping Panel Dryness(TPD) of Hevea brasiliensis causes severe loses to the rubber plantations and leading to severe loss in natural rubber production around the world.To better explore and understand the mechanism of Tapping Panel Dryness(TPD) of Hevea brasiliensis onset,differential proteomic analysis is conducted in lutoid proteins from latex of healthy and TPD trees by two-dimension gel electrophoresis(2-DE).The lutoid proteins from latex of healthy and TPD trees were isolated by TCA/cold acetone precipitation. And these were prepared by means of immobilized pH gradient(IPG) based on 2-DE.After silver staining,the differential expression proteins of two kinds were analyzed using image analysis software(PDQuest).Using proper method stated above,satisfactory 2-DE maps of lutoid proteins from latex of healthy and TPD trees obtained and the preliminary analysis results were reported..There were 13 spots were notable detected,including 10 up-regulated proteins and 3 down-regulated proteins and some different-expressed protein spots between the two types of tissues were detected by mass spectrometry.According to the functions of osmotin in which down-regulated in TPD trees,this protein change might be considered to have relationship with TPD broom.