| 苦楝贮藏蛋白的初步研究 |
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| 引用本文： | 孟春晓,高政权.苦楝贮藏蛋白的初步研究[J].北方园艺,2009(9). |
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| 作者姓名： | 孟春晓 高政权 |
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| 作者单位： | 山东理工大学,生命科学学院,山东,淄博,255049 |
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| 基金项目： | 国家自然科学基金资助项目，山东理工大学博士启动基金资助项目，山东理工大学自然科学基金资助项目 |
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| 摘 要： | 以不同季节的苦楝枝条为研究对象,利用光学显微技术和SDS-PAGE技术研究其枝条中贮藏蛋白在休眠期和生长期的分布情况.经汞-溴酚蓝染色后的石蜡切片显微观察表明,休眠期枝条细胞的液泡中存在大量蓝色近椭圆状蛋白颗粒,而在生长期枝条细胞的液泡中则未发现这种蛋白颗粒的存在.SDS-PAGE结果表明:休眠期枝条中的蛋白条带数目明显多于生长期枝条且浓度高,有12、30、32、38、40、53kD 6条蛋白带在休眠期枝条细胞中大量特异表达.
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| 关 键 词： | 苦楝 贮藏蛋白 显微观察 |
Study of Vegetative Storage Protein in Melia azedarach L. |
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| MENG Chun-xiao,GAO Zheng-quan.Study of Vegetative Storage Protein in Melia azedarach L.[J].Northern Horticulture,2009(9). |
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| Authors: | MENG Chun-xiao GAO Zheng-quan |
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| Institution: | College of Life Sciences;Shandong University of Technology;Zibo;Shandong 255049;China |
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| Abstract: | Vegetative storage proteins(VSPs)in the Melia azedarach L.were identified by using optical microscopy and SDS-PAGE.Under optical microscope,deep blue masses in a granular appearance were observed in the secondary xylem parenchyma cells of the dormancy season barks after stained with mercury-bromophenol blue.However,there was no granular protein in the development season barks.Compared with SDS-PAGE results of the development season barks,there were six 12,30,32,38,40,53 kD major proteins expressed in the do... |
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| Keywords: | SDS-PAGE |
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