番茄再生体系的建立

以"田园6号"番茄子叶和下胚轴为试材,研究激素的种类和浓度对愈伤组织诱导、愈伤组织继代、不定芽分化及生根的影响,并研究了各因素对番茄愈伤组织褐变的影响。结果表明:MS+IBA0.8mg/L+6-BA2.0mg/L诱导所得愈伤最好,MS+IBA1.0mg/L+6-BA0.5mg/L最适于愈伤组织的继代,MS+IAA0.5mg/L+6-BA2.0mg/L和MS+IAA0.5mg/L+6-BA1.0mg/L分别在诱导外质体直接分化不定芽和愈伤组织分化不定芽上效果最佳,MS+IAA0.5mg/L是诱导不定芽生根的理想培养基。外植体种类和光照强度均对愈伤组织的褐化有一定的影响,10mg/L的VC和0.3%的活性炭则能减缓愈伤组织的褐化。

Study on Regeneration System of Lycopersicon esculintum Mill

The cotyledons and hypocotyls of Tianyuan No.6tomato were used as explants to study the effects of the kind of different hormones and their combinations on callus induction,callus subculture,differentiation of adventitious buds and roots and the impact of factors on the tomato callus browning.The results showed that medium of MS+IBA 0.8 mg/L+6-BA 2.0mg/L could induce the best callus,the medium of MS+IBA 1.0mg/L+6-BA 0.5mg/L was most suitable for callus subculture,the mediums of MS+IAA 0.5mg/L+6-BA 2.0mg/L a...