| 姜黄愈伤组织再生体系的建立 |
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| 引用本文： | 张施君,刘念,盛爱武,吴国江.姜黄愈伤组织再生体系的建立[J].北方园艺,2012(6):96-98. |
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| 作者姓名： | 张施君 刘念 盛爱武 吴国江 |
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| 作者单位： | 1. 仲恺农业工程学院园艺与园林学院,广东广州,510225
2. 华南植物园,广东广州,510650 |
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| 基金项目： | 广东省科技攻关资助项目(2006B20201044);中科院百人计划资助项目 |
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| 摘 要： | 以姜黄无菌芽的芽基为外植体,进行愈伤组织的诱导和无菌苗再生的研究。结果表明:在MS培养基中添加2,4-D或2,4-D与6-BA的激素组合能够诱导外植体的切口部位产生愈伤组织,但愈伤生长缓慢;在2,4-D和6-BA的激素组合中再加入TDZ,愈伤组织能够不断增殖生长:在MS+0.5mg/L TDZ+0.5mg/L BA+0.3mg/L 2,4-D培养基中,愈伤的诱导率和生长量最高,诱导率达到80%,每个月愈伤生长量为148.6mg;愈伤组织再分化出芽的适宜培养基为MS+0.5mg/L TDZ+2.0mg/L BA+0.2mg/L NAA,再分化率为90%,外植体的平均出芽数为5.1。当试管苗长至5cm时出瓶移栽,成活率可达90%以上。
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| 关 键 词： | 南昆山莪术 组织培养 芽基 愈伤 再生 |
Establishment of Calli-mediated Regeneration System in Curcuma longa |
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| ZHANG Shi-jun
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LIU Nian
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SHENG Ai-wu
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WU Guo-jiang.Establishment of Calli-mediated Regeneration System in Curcuma longa[J].Northern Horticulture,2012(6):96-98. |
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| Authors: | ZHANG Shi-jun LIU Nian SHENG Ai-wu WU Guo-jiang |
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| Institution: | 1.College of Horticulture and Landscape Architecture,Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong 510225;2.South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,Guangdong 510650) |
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| Abstract: | A protocol was investigated for callus induction using bud base from tissue-cultured Curcuma longa explants.The results showed that MS media supplemented with 2,4-D or with 2,4-D+6-BA could successfully induce calli formation at cut positions of explants.However,the calli grew slowly.The optimal calli induction medium was MS+0.5 mg/L TDZ+0.5 mg/L BA+0.3 mg/L 2,4-D,which showed the highest induction rate and calli growing mass,with an inducing rate of 80% and growing mass of 148.6 mg per month.The optimal plantlet regeneration medium was MS+0.5 mg/L TDZ+2.0 mg/L BA+0.2 mg/L NAA,with a regeneration rate of 90% and the average shoot number of 5.1 per explant.Regenerated plantlets(5 cm at length) were transferred to soil in greenhouse.More than 90% of plantlets could survive. |
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| Keywords: | Curcuma longa tissue culture bud base calli regeneration |
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