库拉索芦荟组织培养细胞分化与芦荟素产生相关性研究

以库拉索芦荟叶为外植体,诱导出愈伤组织.应用电子显微镜观察了愈伤组织培养细胞结构分化状况.用高效液相色谱法测定了培养物中芦荟素的含量.在此基础上探讨了芦荟组织培养细胞分化程度与芦荟素等有效成分产生的关系.结果表明:以叶为外植体,MS NAA1 mg/L 6-BA 0.5 mg/L培养基适于愈伤组织诱导和生长.细胞分化程度很低的愈伤细胞中芦荟素含量很低.细胞高度液泡化、分化程度高的愈伤组织中芦荟素含量较高.植物生长调节物通过影响芦荟愈伤组织细胞分化程度,进而影响芦荟愈伤组织中芦荟素等有效成分的产生.芦荟素含量与愈伤组织中细胞结构分化有密切关系.

Relationship Between Cell Differentiation and Aloin Production in the Callus of Aloe Vera.

The callus was induced from the leaf of Aloe vera.and the cell differentiation of the callus was observed with transmission electronmicroscopy(TEM).The aloin content in callus was determined bymeans of high performance liquid chromatography(HPLC).Based on this result,compared the relations with the extent of cell differentiation of callus and the production of aloin.The results showed it was appropriate for the callus to be induced and growing on the medium ofMS NAA 1 mL/L 0.5 mg/L.The lower the extents of cell differentiation of the callus were,the lower aloin contents were.Thus,firstly plant growth regulators affected the extents of cell differentiation of the callus and further affected the production of aloin.In a word,there were considerable relations between aloin content and cell differentiation of the callus.