| 加拿大铁杉不定芽诱导及植株再生技术研究 |
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| 引用本文： | 刘宝光,田春雨,徐广云.加拿大铁杉不定芽诱导及植株再生技术研究[J].北方园艺,2010(19). |
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| 作者姓名： | 刘宝光 田春雨 徐广云 |
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| 作者单位： | 北华大学林学院采育林创新中心;吉林市林业局林业总站;永吉县金家林业工作站; |
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| 摘 要： | 以加拿大铁杉成熟的合子胚为外植体,进行了组织培养研究。成功地建立了加拿大铁杉的无性繁殖体系。结果表明:适宜愈伤组织诱导的培养基为,改良P6+BA 2.5 mg/L+KT1.0 mg/L+NAA 0.5 mg/L+2,4-D 1.0 mg/L,愈伤组织诱导率达91.5%;适宜不定芽分化的培养基为改良P6+BA 2.8 mg/L+2,4-D 0.125 mg/L,不定芽分化率达4.93个/g;适宜不定芽伸长的培养基为不加任何激素的改良P6培养基,不定芽伸率达34.5%;适宜生根诱导的培养基为:改良1/2 P6+NAA 0.05 mg/L+IBA 0.05 mg/L,生根率达20.5%;适宜练苗的基质为珍珠岩,成活率达28.4%。
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| 关 键 词： | 加拿大铁杉 组织培养 诱导 |
Study on Induction of Adventitious Buds and Plantlet Regeneration of Tsuga canadensis |
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| LIU Bao-guang,TIAN Chun-yu,XU Guang-yun.Study on Induction of Adventitious Buds and Plantlet Regeneration of Tsuga canadensis[J].Northern Horticulture,2010(19). |
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| Authors: | LIU Bao-guang TIAN Chun-yu XU Guang-yun |
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| Institution: | LIU Bao-guang1,TIAN Chun-yu2,XU Guang-yun3(1.Cutting and Culturing Forest Innovation Center of Jilin Province,Forest College of Beihua University,Jilin,Jilin 132013,2.Forestry Central Station of Jilin City Forestry Bureau,Jinjia Forestry Station of Yongji County Forestry Bureau,Yongji,Jilin 132217) |
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| Abstract: | Using mature zygotic embryos of Tsuga canadensis as explants,the tissue culture was studied.The vegetative propagation system was successful established.The results showed that P6 medium supplemented with BA 2.5 mg/L,KT 1.0 mg/L,NAA 0.5 mg/L and 2,4-D 1.0 mg/L was suitable for callus induction and the rate was 91.5%.The improved P6 medium supplemented with BA 2.8 mg/L,2,4-D 0.125 mg/L was suitable for adventitious bud differentiation induction and the rate was 4.93/g.The improved P6 medium supplemented with... |
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| Keywords: | Tsuga canadensis tissue culture induction |
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