|
|||||
|
|
| 圆叶椒草的组织培养与植株再生研究 | |
| 引用本文： | 纪春艳,李睿怡,张彩丽,王詝.圆叶椒草的组织培养与植株再生研究[J].北方园艺,2011(24):138-140. |
| 作者姓名： | 纪春艳 李睿怡 张彩丽 王詝 |
| 作者单位： | 1. 牡丹江师范学院生命科学与技术学院,黑龙江 牡丹江,157012 2. 东北农业大学园艺学院,黑龙江 哈尔滨,150030 3. 齐齐哈尔市甘南二中,黑龙江 甘南,162100 |
| 基金项目： | 黑龙江省教育厅科技面上资助项目(11551516) |
| 摘 要： | 以圆叶椒草茎段为试材,采用植物组织培养方法,研究了6-BA、NAA、IBA对圆叶椒草的愈伤组织诱导、不定芽分化以及试管苗生根培养的影响,以期筛选出愈伤组织和芽分化以及生根诱导的最佳培养基.结果表明:愈伤组织诱导、愈伤组织和不定芽分化最理想的培养基为MS+6-BA 1.0 mg/L+NAA 0.1mg/L;试管苗生根培养最佳培养基为1/2MS+NAA 0.5mg/L+0.5％活性炭. |
| 关 键 词： | 圆叶椒草 茎段 愈伤组织 |
Research on Tissue Culture and Plant Regeneration of Peperomia obtusifolia |
|
| JI Chun-yan , LI Rui-yi , ZHANG Cai-li , WANG Zhu.Research on Tissue Culture and Plant Regeneration of Peperomia obtusifolia[J].Northern Horticulture,2011(24):138-140. | |
| Authors: | JI Chun-yan LI Rui-yi ZHANG Cai-li WANG Zhu |
| Institution: | JI Chun-yan1,LI Rui-yi2,ZHANG Cai-li3,WANG Zhu1(1.Institute of Life Science and Technology,Mudanjiang Normal University,Mudanjiang,Heilongjiang 157012;2.Institute of Horticultural,Northeast Agricultural University,Harbin,Heilongjiang 150030;3.Qiqihar Gannan No.2 Middle School,Gannan,Heilongjiang 162100) |
| Abstract: | Taking the round leaf segments of pepper stems as test materials,the effect of 6-BA,NAA,IBA on pepper grass callus induction,adventitious bud differentiation and plantlet rooting were studied,to filter out the callus and shoot induction of differentiation and the best rooting medium.The results showed that MS+1.0 mg/L+NAA 0.1 mg/L for the conduct of callus induction,callus and adventitious buds optimal differentiation medium,1/2MS+NAA 0.5 mg/L+0.5% activated carbon were the best medium for in vitro rooting culture. |
| Keywords: | Peperomia obtusifolia caulis segment tissue culture |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |