| 肉苁蓉种内变异的AFLP标记研究 |
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| 引用本文： | 甘晓燕,李苗,马洪爱,宋玉霞.肉苁蓉种内变异的AFLP标记研究[J].北方园艺,2011(3):141-143. |
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| 作者姓名： | 甘晓燕 李苗 马洪爱 宋玉霞 |
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| 作者单位： | 宁夏农林科学院,农业生物技术重点实验室,宁夏,银川,750002;宁夏农林科学院,农业生物技术重点实验室,宁夏,银川,750002;宁夏农林科学院,农业生物技术重点实验室,宁夏,银川,750002;宁夏农林科学院,农业生物技术重点实验室,宁夏,银川,750002 |
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| 基金项目： | 国家自然科学基金，宁夏自然基金 |
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| 摘 要： | 利用AFIP标记方法对肉苁蓉(Cistanche deserticola Y.C.Ma)种内不同花冠类型进行分子标记鉴定。结果表明:从64对引物组合中选出扩增带纹丰富清晰,且多态性较好的引物组合15对,共扩增出条带266条,多态性条带148条,多态性位点百分比为55.6%。从花冠裂片类型可将肉苁蓉划分为9种不同类型,9种类型遗传距离的变异范围在0.1778～0.8462之间。
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| 关 键 词： | 肉苁蓉 AFLP 变异类型 |
Study on Intraspecific Variations of Cistanche deserticola Y.C.Ma Using AFLP Molecular Markers |
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| GAN Xiao-yan,LI Miao,MA Hong-ai,SONG Yu-xia.Study on Intraspecific Variations of Cistanche deserticola Y.C.Ma Using AFLP Molecular Markers[J].Northern Horticulture,2011(3):141-143. |
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| Authors: | GAN Xiao-yan LI Miao MA Hong-ai SONG Yu-xia |
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| Institution: | GAN Xiao-yan,LI Miao,MA Hong-ai,SONG Yu-xia (Key Lab of Agricultural Biotechnology,Yinchuan,Ningxia 750002) |
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| Abstract: | Identification of Cistanche deserticola relationship using AFLP.The results showed that the from 64 primer combinations,15 polymorphic primer combinations were seleted to perform the AFLP.266 bands have been amplified altogether,among which 148 bands showed polymorphism,amounting to 55.6%.Cistanche deserticola Y.C.Ma was divided 9 types from the types of corolla the genetic distances varied from 0.1778～0.8462. |
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| Keywords: | AFLP |
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