| 紫叶稠李叶片离体培养再生植株 |
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| 引用本文： | 邢国杰,高明,谭化,李俊波,崔云志,王中伟.紫叶稠李叶片离体培养再生植株[J].北方园艺,2011(3):166-168. |
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| 作者姓名： | 邢国杰 高明 谭化 李俊波 崔云志 王中伟 |
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| 作者单位： | 吉林省农业科学院生物技术中心,吉林,长春,130124 |
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| 摘 要： | 以紫叶稠李为试材,采用组织培养方法,研究不同激素对其试管苗不定芽诱导、不定芽生根及成苗影响。结果表明:叶片切块可诱导产生大量的不定芽,MS附加0.5mg/L BA和0.01mg/L NAA的培养基不定芽诱导率为50.1%;利用植物激素0.5mg/L BA,0.5mg/LNAA,0.5mg/L KT和0.2mg/L IBA的组合,不定芽可多次继代培养增殖;不定芽在附加0.4mg/L IBA的MS培养基上生根,生根率为67%。该试验结果说明,紫叶稠李试管苗叶片可作为外植体离体培养获得再生植株,BA是主要的调控植物激素,IBA对生根有一定促进作用。
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| 关 键 词： | 紫叶稠李 不定芽 离体再生 |
Study on Regeneration Plant of Prunus Wilsonil Leaves in vitro |
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| XING Guo-jie,GAO Ming,TAN Hua,LI Jun-bo,CUI Yun-zhi,WANG Zhong-wei.Study on Regeneration Plant of Prunus Wilsonil Leaves in vitro[J].Northern Horticulture,2011(3):166-168. |
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| Authors: | XING Guo-jie GAO Ming TAN Hua LI Jun-bo CUI Yun-zhi WANG Zhong-wei |
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| Institution: | XING Guo-jie,GAO Ming,TAN Hua,LI Jun-bo,CUI Yun-zhi,WANG Zhong-wei (Bio-technique Research Center,Jilin Academy of Agricultural Sciences,Changchun,Jilin 130124) |
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| Abstract: | Rapid propagation of Prunus Wilsonil was preliminary stuidied using leaves of testtube seedling for in vitro culture.The results showed that the a chip of leaf from test-tube plantlet were cultured,a mass of adventitious shoots were induced with MS medium containing BA 0.5 mg/L + NAA 0.01 mg/L,its induction rate was 50.1%;and combination with BA 0.5 mg/L + NAA 0.5 mg/L + KT 0.5 mg/L + IBA 0.2 mg/L,adventitious shoots could be used repeatedly to subculture proliferation:rooting was induced with adventitious ... |
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| Keywords: | Prunus Wilsonil adventitious shoot regeneration in vitro |
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