| 菊花离体快繁技术研究 |
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| 引用本文： | 龚明霞,陈小凤,方锋学,黄熊娟,梁家作.菊花离体快繁技术研究[J].北方园艺,2010(1):172-173. |
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| 作者姓名： | 龚明霞 陈小凤 方锋学 黄熊娟 梁家作 |
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| 作者单位： | 广西农业科学院,蔬菜研究所,广西,南宁,530007;广西农业科学院,蔬菜研究所,广西,南宁,530007;广西农业科学院,蔬菜研究所,广西,南宁,530007;广西农业科学院,蔬菜研究所,广西,南宁,530007;广西农业科学院,蔬菜研究所,广西,南宁,530007 |
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| 基金项目： | 广西农业科学院科技发展基金资助项目(2007010) |
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| 摘 要： | 以菊花‘神马’和‘黄绣球’的茎尖为外植体进行组织培养,研究了基因型及激素组合对不定芽分化、增殖及生根的影响。结果表明:最适诱导分化培养基为:‘神马’:MS+6-BA 1 mg/L+NAA 0.1 mg/L;‘黄绣球:’MS+6-BA 2 mg/L+NAA 0.1 mg/L。最适增殖培养基为:‘神马:’MS+6-BA 1 mg/L+NAA 0.2 mg/L;‘黄绣球:’MS+6-BA 1 mg/L+NAA 0.5 mg/L。两者的最适生根培养基都为:1/2MS+NAA 0.2 mg/L。
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| 关 键 词： | 菊花 茎尖培养 激素 |
Research on Rapid Propagation Technology of Famous Chrysanthemum in vitro |
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| GONG Ming-xia,CHEN Xiao-feng,FANG Feng-xue,HUANG Xiong-juan,LIANG Jia-zuo.Research on Rapid Propagation Technology of Famous Chrysanthemum in vitro[J].Northern Horticulture,2010(1):172-173. |
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| Authors: | GONG Ming-xia CHEN Xiao-feng FANG Feng-xue HUANG Xiong-juan LIANG Jia-zuo |
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| Institution: | Vegetable Research Institute of Guangxi Academy of Agricultural Sciences;Nanning;Guangxi 530007 |
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| Abstract: | Using the stem stips of two chrysanthemum cultivars as explants for tissue culture,the effect of genotype and hormone combinations on differentiation,proliferation and rooting of shoots were investigated.The results showed that optimized differentiation media respectively were:'Jinba':MS+6-BA 1 mg/L+NAA 0.1 mg/L;'Yellow Embroidered Ball':MS+6-BA 2 mg/L+NAA 0.1 mg/L.Optimized proliferation media respectively were:'Jinba': MS+6-BA 1 mg/L+NAA 0.2 mg/L;'Yellow Embroidered Ball':MS+6-BA 1 mg/L+NAA 0.5 mg/L.Optim... |
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| Keywords: | chrysanthemum stem tip culture hormone |
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