| 白菜型油菜高效离体再生体系的建立 |
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| 引用本文： | 裴冬丽.白菜型油菜高效离体再生体系的建立[J].北方园艺,2011(1):127-129. |
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| 作者姓名： | 裴冬丽 |
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| 作者单位： | 商丘师范学院生命科学系,植物与微生物互作重点实验室,河南商丘,476003;河南农业大学农学院,河南郑州,450002 |
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| 基金项目： | 河南省科技发展计划国际合作资助项目(084300510076); 河南省科技厅基础与前沿技术资助项目(082300430320) |
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| 摘 要： | 以白菜型油菜(Brassicarapa)的亚种菜心(Brassica parachinensis)的子叶和下胚轴为外植体,研究不同浓度的NAA和6-BA配比对菜心组织培养的影响。结果表明:菜心子叶和下胚轴组织培养的再生最佳培养基分别是MS+6-BA1.5 mg/L+NAA0.2 mg/L+AgNO35 mg/L和MS+6-BA2.0 mg/L+NAA0.2 mg/L+AgNO35 mg/L;不定芽生根最佳培养基是MS+NAA0.2 mg/L。同时还研究了AgNO3浓度对菜心组织培养的影响,在培养基中附加AgNO3能提高菜心芽的诱导频率。
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| 关 键 词： | 白菜型油菜 植株再生 组织培养 |
Establishment of Effective Regeneration System of Brassica rapa in vitro |
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| PEI Dong-li.Establishment of Effective Regeneration System of Brassica rapa in vitro[J].Northern Horticulture,2011(1):127-129. |
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| Authors: | PEI Dong-li |
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| Institution: | PEI Dong-li1,2 (1.Key Laboratory of Plant-Microbe Interactions,Department of Life Science,Shangqiu Normal University,Shangqiu,Henan 47600,2.College of Agronomy,Henan Agricultural University,Zhengzhou,Henan 450002) |
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| Abstract: | Cotyledons and hypocotyls of Brassica parachinensis subspecies of Brassica rapa were used as explants to study the effect of different concentration ratio of NAA and 6-BA on Brassica parachinensis tissue culture.The results showed that the best regeneration medium for cotyledons and hypocotyls of Brassica parachinensis were MS+6-BA 1.5 mg/L+NAA 0.2 mg/L+AgNO3 5 mg/L and MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+AgNO3 5 mg/L respectively.The best root medium of adventitious buds was MS+NAA 0.2 mg/L.It was also discussed... |
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| Keywords: | Brassica rapa plant regeneration tissue culture |
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