乌饭树组织培养技术

以乌饭树嫩枝作为外植体,对乌饭树组织培养技术中无菌外植体获得、不定芽产生、生根培养等步骤进行研究,以期建立乌饭树组培快繁技术。结果表明:最有效的消毒方法是用0.1%氯化汞浸泡外植体10 min。WPM基本培养基适合诱导乌饭树外植体不定芽的产生,MS基本培养基不适合诱导乌饭树外植体产生不定芽。WPM+ZT 2.0 mg·L^-1培养基上的不定芽诱导率最高,为97.7%。添加马铃薯切块的培养基明显促进了丛生不定芽的伸长,因此适合丛生不定芽伸长的培养基为WPM+ZT 1.0 mg·L^-1+马铃薯切块100 g·L^-1。已伸长的芽转移至1/2WPM+NAA 1.0 mg·L^-1+活性炭500 mg·L^-1培养基上生根效果较好,6周后可达到94.7%的生根率。

Key Techniques in Tissue Culture With Vaccinium bracteatum

Shoots of Vaccinium bracteatum were used as explants to research the key points in tissue culture,which referring to how to obtain sterile explants,how to generate adventitious buds,and how to rooting,in order to established tissue culture of Vaccinium bracteatum.The results showed that the most effective way to disinfect explants in this experiment was soaking them into 0.1% mercury chloride solution for 10 minutes.It was suitable for inducing adventitious buds of Vaccinium bracteatum in WPM basic medium while it was not suitable for inducing adventitious buds of Vaccinium bracteatum in MS basic medium.The highest induction rate of adventitious buds was97.7%,and the medium was WPM+ZT 2.0 mg·L^-1.The medium in which the potato diced was added significantly promoted the elongation of the cluster adventitious buds,so the medium suitable for the elongation of the adventitious buds was WPM+ZT 1.0 mg·L^-1+potato diced 100 g·L^-1.The rooting effect of the elongated buds was better with 1/2 WPM + NAA 1.0 mg·L^-1+ activated carbon 500 mg·L^-1 medium,a rooting rate of 94.7%could be achieved after 6 weeks.