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风信子离体快繁技术研究
引用本文:刘爽,孙余丹,李叶青.风信子离体快繁技术研究[J].北方园艺,2011(20):137-139.
作者姓名:刘爽  孙余丹  李叶青
作者单位:湛江师范学院生命科学与技术学院,广东湛江,524048;湛江师范学院生命科学与技术学院,广东湛江,524048;湛江师范学院生命科学与技术学院,广东湛江,524048
摘    要:利用离体快繁技术,以风信子的鳞茎为外植体,添加不同浓度6-BA和NAA进行不定芽的诱导、不定芽的继代和生根培养.结果表明:鳞片的初代最佳诱导培养基为:MS+6-BA 1.5 mg/L+NAA 0.3 mg/L,诱导率达到80%;最佳继代培养基为:MS+6-BA 1.0 mg/L+NAA 0.2 mg/L;生根诱导最佳培养基:1/2MS+NAA0.3 mg/L,生根率可达90%.

关 键 词:风信子  鳞茎  不定芽  6-BA  NAA

Study on the Rapid Propagation in vitro of Common Hyacinthus
LIU Shuang , SUN Yu-dan , LI Ye-qing.Study on the Rapid Propagation in vitro of Common Hyacinthus[J].Northern Horticulture,2011(20):137-139.
Authors:LIU Shuang  SUN Yu-dan  LI Ye-qing
Institution:LIU Shuang,SUN Yu-dan,LI Ye-qing(School of Bioscience and Technology,Zhanjiang Normal College,Zhanjiang,Guangdong 524048)
Abstract:From the body of rapid propagation technique,taking bulb of Hyacinthus as explant addition different of 6-BA and NAA,directly in vitro shoot induction,in vitro shoot subculture multiplication and root of training.The results showed that the scales at best and inductive medium was MS+6-BA 1.5 mg/L+NAA 0.3 mg/L,induction rate of 80%;The best medium on subculture multiplication was MS+6-BA 1.0 mg/L+NAA 0.2 mg/L;Take root induction medium:1/2 best MS with NAA 0.3 mg/L,rooting rate coul amount to 90%.
Keywords:common Hyacinthus  scale  adventitious bud  6-BA  NAA  
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