| 甘蓝胞质雄性不育系育性相关线粒体DNA片断的克隆及序列分析 |
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| 引用本文： | 许忠民,张恩慧,巩振辉,程永安,马青山.甘蓝胞质雄性不育系育性相关线粒体DNA片断的克隆及序列分析[J].北方园艺,2011(21):106-110. |
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| 作者姓名： | 许忠民 张恩慧 巩振辉 程永安 马青山 |
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| 作者单位： | 西北农林科技大学园艺学院,陕西杨凌,712100;西北农林科技大学园艺学院,陕西杨凌,712100;西北农林科技大学园艺学院,陕西杨凌,712100;西北农林科技大学园艺学院,陕西杨凌,712100;西北农林科技大学园艺学院,陕西杨凌,712100 |
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| 基金项目： | 国家“十一五”科技支撑计划资助项目(2008BADB1B02); 陕西省农业科技攻关计划资助项目(2009K01-24); 西安市农业科技攻关计划资助项目(NC09041(1)); 西北农林科技大学唐仲英植物育种专项资助项目 |
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| 摘 要： | 以甘蓝结球期叶球内叶为试材,利用PCR方法在甘蓝新型胞质雄性不育材料CMS451的线粒体基因组中扩增出与细胞质雄性不育相关的片断,并克隆测序.结果表明:该片断和NCBI发布的Ogura胞质不育系特异片段高度同源,说明CMS451的不育机理与Ogu-CMS相似,也反映了CMS基因的相对保守性.
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| 关 键 词： | 甘蓝 CMS451 细胞质雄性不育(CMS) 线粒体DNA(mtDNA) |
Cloning and Sequencing of a Mitochondrial DNA Fragment Related to Cytoplasmic Male Sterile Line of Cabbage |
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| XU Zhong-min
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ZHANG En-hu
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GONG Zhen-hui
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CHENG Yong an
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MA Qing-shan.Cloning and Sequencing of a Mitochondrial DNA Fragment Related to Cytoplasmic Male Sterile Line of Cabbage[J].Northern Horticulture,2011(21):106-110. |
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| Authors: | XU Zhong-min ZHANG En-hu GONG Zhen-hui CHENG Yong an MA Qing-shan |
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| Institution: | XU Zhong-min,ZHANG En-hui,GONG Zhen-hui,CHENG Yong-an,MA Qing-shan(College of Horticulture,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100) |
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| Abstract: | Taking ball leaves of cabbage in the period of ball as test materials,the special sequence related to CMS was amplified in the new e cabbage CMS451 by PCR,and its homology was highly with Ogu-CMS published in NCBI.The results showed that CMS451 had the similar sterility with Ogu-CMS,but the gene related to CMS was relative conservative. |
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| Keywords: | cabbage CMS451 cytoplasmic male sterility(CMS) mitochondrial DNA(mtDNA) |
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