| 响应面法优化软枣猕猴桃枝条多糖提取工艺 |
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| 引用本文： | 牛强,刘悦,刘畅,孙宏莱,N.V.斯克里普琴科,刘德江.响应面法优化软枣猕猴桃枝条多糖提取工艺[J].北方园艺,2020(9):118-124. |
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| 作者姓名： | 牛强 刘悦 刘畅 孙宏莱 N.V.斯克里普琴科 刘德江 |
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| 作者单位： | 佳木斯大学药学院;中-乌农林技术开发与应用国际合作联合实验室;乌克兰国家科学院M.M.格里什科国家植物园 |
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| 基金项目： | 佳木斯大学校长创新创业基金资助项目(XZYF2019-18);科技部高端外国专家引进计划资助项目(G20190211004);中央财政支持地方高校发展基金资助项目;北药与功能食品特色学科建设资助项目。 |
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| 摘 要： | 以软枣猕猴桃枝条为试材,通过响应面法优化软枣猕猴桃枝条多糖(polysaccharides from the branches of Actinidia arguta)的提取工艺,采用苯酚-硫酸法对软枣猕猴桃枝条多糖定量分析,在单因素试验的基础上,利用响应面法对提取软枣猕猴桃枝条多糖的各个因素进行优化。结果表明:响应面法优化多糖提取的最佳工艺为液料比29∶1 mL·g^-1,提取温度80℃,提取时间128 min,在最优条件下多糖的平均提取率为3.31%。该研究采用响应面法对软枣猕猴桃枝条多糖的提取条件优化合理,为今后软枣猕猴桃的开发利用提供参考依据。
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| 关 键 词： | 软枣猕猴桃枝条 多糖 响应面优化 提取 |
Optimization of Extraction Technology of Polysaccharides From the Branches of Actinidia arguta by Response Surface Methodology |
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| NIU Qiang,LIU Yue,LIU Chang,SUN Honglai,N.V.Skrypchenko,LIU Dejiang.Optimization of Extraction Technology of Polysaccharides From the Branches of Actinidia arguta by Response Surface Methodology[J].Northern Horticulture,2020(9):118-124. |
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| Authors: | NIU Qiang LIU Yue LIU Chang SUN Honglai NVSkrypchenko LIU Dejiang |
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| Institution: | (College of Pharmacy,Jiamusi University,Jiamusi,Heilongjiang 154007;China-Ukraine Agriculture&Forestry Technology Development and Application International Cooperation Joint Lab,Jiamusi,Heilongjiang 154007;M.M.Gryshko National Botanical Gardens of National Academy of Sciences of Ukraine,Kyiv,Ukraine 01014) |
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| Abstract: | Using the branches of Actinidia arguta as the raw material to optimize the extraction technology of polysaccharides from the branches of Actinidia arguta(Sieb.et Zucc.)Planch.ex Miq.by response surface methodology(RSM).Phenol-sulfuric acid method was used to quantitatively analyze.On the basis of single factor experiment,response surface methodology was applied to optimize the main extraction conditions.The best conditions for the response surface optimization were as follows,liquid-material ratio 29∶1 mL·g^-1,extraction temperature 80℃,and extracting time 128 minutes.The extraction rate of polysaccharide was 3.31%under the optimal conditions.The extraction conditions of polysaccharides from the branches of Actinidia arguta(Sieb.et Zucc.)Planch.ex Miq.were optimized by response surface method.This result could provide a theoretical basis for the further exploitation and utilization of Actinidia arguta. |
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| Keywords: | Actinidia arguta branch polysaccharides response surface method optimization extraction |
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