菠萝蜜遗传多样性的ISSR分析

用ISSR标记方法对76份菠萝蜜(Artocarpus heterophyllus Lam.)种质资源DNA的遗传多样性进行了检测,24个引物共检测到477条带,其中427条具多态性(占89.52%),平均PIC为0.23,24个引物能把76份种质完全区分开来。遗传距离分析结果显示供试种质的遗传多样性较低,在DNA水平上的遗传相似系数为0.626～0.945,平均为0.775。聚类分析表明,76份菠萝蜜材料在遗传距离系数为0.752处可分为4大类,其中热带植物园的种质WJ2和GSYWJ1与其他种质的遗传距离相对较大,干胞和湿类型不能独立聚类。另外各地区的种质与雷州半岛种质明显分开聚类,而雷州半岛各地区的种质混杂聚类在一起,不能按地区单独聚类。

Analysis of genetic diversity of Jackfruit germplasm using ISSR marking method

ISSR(inter-simple sequence repeat)markers were used to analyze genetic diversity in 76 accessions of jackfruit(Artocarpus heterophyllus Lam.).Among 477 bands produced by 24 ISSR primers,427 were polymorphic(accounted for 89.52%).The average PIC(polymorphism information content) was 0.23.These 76 jackfruit accessions could be discriminated by the 24 ISSR primers.Genetic distance analysis revealed that, low genetic diversity existed in the jackfruit germplasm studied,and the genetic similarity coefficient ranged from 0.626 to 0.945,with an average of 0.775.Cluster analy-sis showed that 4 groups could be clustered at a genetic distance coefficient of 0.752.The results indicated that,2 accessions from Tropical Plant Garden,WJ2 and GSYWJ1,were genetically different from other accessions.Accessions of soft flesh type and firm flesh type could not be discriminated.Accessions collected from different sources could form independent clusters, while accessions collected from different areas of Leizhou peninsula could not form independent clusters.