| 香榧性别鉴定的RAPD和SCAR标记 |
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| 引用本文： | 戴正,陈力耕,童品璋.香榧性别鉴定的RAPD和SCAR标记[J].果树学报,2008,25(6):856-859. |
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| 作者姓名： | 戴正 陈力耕 童品璋 |
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| 作者单位： | [1]浙江大学农业与生物技术学院园艺系,杭州310029 [2]青岛农业大学园艺学院,山东青岛266109 [3]浙江省诸暨市林业局,浙江诸暨311800 |
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| 摘 要： | 为了对香榧幼苗进行早期的性别鉴定,利用RAPD标记技术对香榧品种中的雌性和雄性群体进行了分析。在300条RAPD随机引物中,用引物S250扩增得到了1条大约600bp雄性特异性片段,将其命名为S250-600。进一步对该RAPD标记片段进行回收、克隆和测序,获得了593bp的该标记的核苷酸特定序列。根据对该片段的序列分析结果,设计了1对引物,成功地将RAPD标记转化为SCAR标记,命名为SW-593。将该序列递交至GenBank数据库,接收号为:EU670673。利用该SCAR标记对香榧的雌性和雄性个体进行性别鉴定,结果表明,该SCAR标记在雄性个体中均能稳定出现,说明此SCAR标记是一个与香榧雄性基因连锁的标记,可以用来对香榧品种进行大规模的性别鉴定。
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| 关 键 词： | 香榧 性别鉴定 RAPD SCAR |
RAPD and SCAR molecular markers for sex identification in Torreya grandis |
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| DAI Zheng,CHEN Li-geng,TONG Pin-zhang.RAPD and SCAR molecular markers for sex identification in Torreya grandis[J].Journal of Fruit Science,2008,25(6):856-859. |
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| Authors: | DAI Zheng CHEN Li-geng TONG Pin-zhang |
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| Institution: | DAI Zheng1,2,CHEN Li-geng1,TONG Pin-zhang3(1Department of Horticulture,Agricultural , Bio-technology College,Zhejiang University,Hangzhou,Zhejiang 310029,China,2Col-lege of Horticulture,Qingdao Agricultural University,Qingdao,Sh,ong 266109 China,3Department of Forestry,Zhuji municipality,Zhejiang 311800 China) |
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| Abstract: | In order to identify the sex in Torreya grandis Fort seedlings,the populations of male and female T.grandis cv.Merrillii were analyzed by RAPD with 300 10-mer primers tested.A male-associated fragment was generated with S250 primer.The length of the fragment was about 600 bp,and was named as S250-600.S250-600 was recovered from electrophoresis gel and ligated into the pUCm-T vector system for being sequenced.The results showed that this marker consisted of 593 bp with specific nucleotide sequence.Two primer... |
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| Keywords: | RAPD SCAR |
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