不同苹果砧木组培苗抗旱性的比较研究

【目的】为了提供苹果耐旱育种及栽培材料,【方法】以5种苹果砧木组培苗为试材,PEG-6000作为胁迫剂,模拟室外干旱进行处理,运用模糊隶属函数抗旱性综合分析,比较了5个苹果砧木组培苗的耐旱性。【结果】结果表明,在PEG-6000胁迫条件下,不同苹果砧木的叶绿素含量总体均呈下降趋势,叶片的相对电导率和MDA含量随PEG-6000胁迫质量分数的升高逐渐升高。在整个胁迫过程中,各苹果砧木组培苗叶片内蛋白质含量随胁迫质量分数的升高而增大。SOD活性随PEG-6000胁迫质量分数的升高呈先升高后下降趋势,且在20%PEG-6000胁迫条件下SOD活性达到了最大。POD活性随胁迫质量分数的升高呈现出逐渐升高的趋势。【结论】5种苹果砧木的抗旱性顺序依次为:新疆野苹果>平邑甜茶>八棱海棠>‘M26’>‘M9’。

Drought resistance of different apple rootstocks in vitro

【Objective】 The study was to provide drought tolerant apple lines for breeding and cultivation practice.【Method】 Tissue cultured shoots of five apple rootstocks were treated with PEG-6000,simulating field drought stress conditions.Drought tolerance of the shoots was evaluated by meta-analysis using fuzzy membership function.【Result】 The results showed that the chlorophyll content displayed a decreasing tendency under PEG-6000 stress,whereas the relative electric conductivity and malondialdehyde(MDA) content in leaf increased gradually with the increase of PEG-6000 concentration.In the stress treatment,the protein content in leaf of all rootstocks increased with the PEG-6000 concentration.Superoxide dismutases(SOD) activity first increased and then declined with increasing PEG-6000 concentration,with a peak value at 20% PEG-6000.However,the POD activity went up gradually in all the rootstocks with the increase of drought stress strength.【Conclusion】 The drought tolerances of the five rootstocks ranked as follows: Malus sieversii>M.hupehensis>M.micromalus>'M26'>'M29'.