| 利用胚性细胞悬浮系研究香蕉枯萎病抗性离体筛选技术 |
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| 引用本文： | 徐春香,陈佳越,潘晓,王泽槐,陈厚彬.利用胚性细胞悬浮系研究香蕉枯萎病抗性离体筛选技术[J].果树学报,2008,25(5):686-690,F0003. |
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| 作者姓名： | 徐春香 陈佳越 潘晓 王泽槐 陈厚彬 |
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| 作者单位： | [1]华南农业大学热带亚热带果树研究室 [2]华南农业大学南方果树生理研究室,广州510642 |
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| 基金项目： | 广东省自然科学基金，广东省科技攻关计划，农业行业专项基金 |
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| 摘 要： | 以香蕉(Musa spp. AAA group)胚性细胞悬浮系(Embryogenic Cell Suspension,ECS)为试材,进行抗枯萎病离体筛选技术研究。首先测试不同的γ射线(60Co)辐射剂量对ECS植株再生能力的影响;在此基础上,以不同浓度的枯萎病病原菌毒素粗滤液(FOC)为选择压力,研究FOC对ECS植株再生能力的影响,并进行离体抗性筛选。结果表明,ECS的植株再生能力与辐射剂量之间呈负相关,半致死剂量为70～80Gy。同样地,未经辐射处理的ECS其植株再生能力也随着FOC体积分数的增加而急剧下降,但经辐射处理过的ECS其植株再生能力与CK相比成倍地增加;当FOC体积分数达到12%后,只有从经80Gy辐射后的ECS获得了再生植株。这表明ECS耐FOC的能力随着辐射剂量的增加而增强。
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| 关 键 词： | 香蕉 辐射 病原菌毒素粗滤液(FOC) 体细胞胚发生 离体筛选 |
Study on in vitro screening technique for mutants resistant to Fusarium wilt through embryogenic cell suspension of banana (Musa spp. AAA group) |
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| XU Chun-xiang,CHEN Jia-yue,PAN Xiao,WANG Ze-huai,CHEN Hou-bin.Study on in vitro screening technique for mutants resistant to Fusarium wilt through embryogenic cell suspension of banana (Musa spp. AAA group)[J].Journal of Fruit Science,2008,25(5):686-690,F0003. |
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| Authors: | XU Chun-xiang CHEN Jia-yue PAN Xiao WANG Ze-huai CHEN Hou-bin |
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| Institution: | XU Chun-xiang1,2,CHEN Jia-yue1,PAN Xiao1,WANG Ze-huai2,CHEN Hou-bin1,2(1Tropical , Subtropical Fruit Research Laboratory,South China Agricultural University,Guangzhou,Guangdong 510642 China,2Physiological Laboratory for South China Fruits,Guangdong 510642 China) |
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| Abstract: | In this study,the embryogenic cell suspension(ECS)of banana(Musa spp.AAA group)was used as material for in vitro screening of clones resistant to Fusarium wilt.The effect of gamma radiation at different dosages on plant regeneration capacity of ECS was firstly investigated.The results showed that the plant regeneration capacity of ECS decreased with the increase in radiation dosage,and it was negatively related to radiation dosages.When the ECS was radiated at 40 Gy,its plant regeneration capacity was highe... |
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| Keywords: | Banana(Musa spp AAA group) Irradiation Pathogenic toxin Somatic embryogenesis In vitro screening |
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