台湾青枣不同品种花粉萌发和生活力测定

以5个台湾青枣品种盛花期花粉为试材，应用正交试验测定不同品种花粉萌发率及适宜的萌发条件，并用4种不同方法测定花粉生活力。结果表明：（1）不同品种的花粉萌发率存在显著差异，其中大世界的花粉萌发率最高。（2）硼酸浓度、培养温度等因素对花粉萌发率有极显著影响。最适培养基为含琼脂1％、蔗糖15％、硼酸0．02％的组合，适宜温度为30—32℃。（3）花粉生活力的测定中，离体萌发法是最佳选择，I—KI染色法、醋酸洋红染色法和TTC染色法不适宜用作台湾青枣花粉生活力的测定。

Examination of pollen germination and vitality of different cultivars of Ziziphus mauritiana in greenhouse

Pollen germination percentage of 5 different cultivars of Zizyphus mauritiana were studied by using the orthogonal experiment design. In vitro culture and 3 staining tests (I-KI,aceto-carmine and TTC) were used to determine the pollen viability. The results showed: (1) pollen germination percentage of the 5 cultivars were significantly different, the Big World had the highest pollen germination percentage; (2) the experimental factors such as pollen cultivars, BA (boric acid) concentration in the medium and temperature significantly affected pollen germination percentage. The most suitable medium was 1% agar + 15% sucrose + 0.02% BA; (3) In vitro culture was the best method for determining pollen viability.