| 中国野生葡萄醛脱氢酶基因在拟南芥中的过量表达 |
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| 引用本文： | 雷龑,王跃进,徐伟荣,张方方,余皓,王西平.中国野生葡萄醛脱氢酶基因在拟南芥中的过量表达[J].果树学报,2009,26(1). |
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| 作者姓名： | 雷龑 王跃进 徐伟荣 张方方 余皓 王西平 |
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| 作者单位： | 农业部西北园艺植物种质资源利用重点开放实验室,陕西省农业分子生物学重点实验室,西北农林科技大学园艺学院,陕西杨凌,712100
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| 摘 要： | 将质粒pWR306中包含ED35s启动子、Omega元件及TNOS终止子的一段核苷酸序列定向克隆到质粒pCAMBIA1300,构建了植物中间表达载体pWR-II;将重组质粒pGEM-T-ALDH的醛脱氢酶(ALDH)基因片段定向克隆到pWR-II,构建了葡萄ALDH基因的植物过量表达载体pWR-II-ALDH。采用改进冻融法将其导入农杆菌EHA105,采用花序浸蘸法转化拟南芥,获得了潮霉素抗性的转化拟南芥植株。PCR检测证明外源基因已整合进拟南芥基因组,Real-timePCR结果表明ALDH基因在转化植株的表达量远高于野生种。转化植株和野生植株在形态上有一定的差异,说明葡萄ALDH基因在拟南芥中表达对其生长发育有一定影响。
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| 关 键 词： | 中国野生葡萄 醛脱氢酶基因 过量表达载体 拟南芥 |
Overexpression in Arabidopsis thaliana with a novel gene involved in ALDH from Chinese wild Vitis pseudoreticulata |
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| LEI Yan,WANG Yue-jin,XU Wei-rong,ZHANG Fang-fang,YU Hao,WANG Xi-ping.Overexpression in Arabidopsis thaliana with a novel gene involved in ALDH from Chinese wild Vitis pseudoreticulata[J].Journal of Fruit Science,2009,26(1). |
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| Authors: | LEI Yan WANG Yue-jin XU Wei-rong ZHANG Fang-fang YU Hao WANG Xi-ping |
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| Institution: | Key Laboratory of Horticultural Plant Germplasm Resources Utilization Northwest in China;Ministry of Agriculture;Key Laboratory of Agriculture Molecular Biology of Shaanxi Province;Horticultural College;Northwest A & F University;Yangling;Shaanxi 712100 China |
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| Abstract: | A nucleotide sequence of plasmid pWR306 carrying promotor ED35s, Omega TMV translation enhancer and nopaline synthase terminator was specifically cloned into plasmid pCAMBIA1300 to construct the plant intermediate expres- sion vector pWR-II; the segment of aldehydehyde dehydrogenase gene from the recombinant plasmid pGEM-T-ALDH was specifically cloned into intermediate expression vector pWR-II to construct the plant overexpression vector pWR-II-ALDH, which was introduced into Agrobacterium strain EHA105 by ... |
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| Keywords: | Chinese wild Vitis pseudoreticulata Aldehydehyde dehydrogenase gene Overexpression vector Arabidopsis thaliana |
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