金丝小枣等4个枣品种耐盐性与细胞质膜、液泡膜质子泵活性的关系

以金丝小枣、梨枣、大瓜枣和冬枣为试材,设加0、0.10%、0.30%、0.50%和0.70%NaCl共5种土壤含盐量水平,研究了盐胁迫下这4个主栽枣品种耐盐性与质膜H+-ATPase和液泡膜H+-ATPase、H+-PPase活性的关系。对叶片相对含水量、丙二醛含量、相对电解质渗漏率、Fv/Fo、Fv/Fm、净光合速率等生理指标测试结果表明,4个枣品种的耐盐性存在较大差异。尽管4品种根尖和叶片细胞PM-H+-ATPase、TP-H+-ATPase和TP-H+-PPase活性随土壤含盐量升高的变化趋势相似,但同一枣品种的不同H+-ATPase、同一H+-ATPase在不同枣品种中的活性差异明显。盐胁迫下,耐盐性强的金丝小枣根尖细胞中3种H+-酶的活性降低幅度明显小于耐盐性弱的冬枣,且金丝小枣PM-H+-ATPase活性与TP-H+-ATPase活性相当,而盐胁迫下耐盐性弱的冬枣根尖细胞中PM-H+-ATPase活性明显低于TP-H+-ATPase活性,TP-H+-PPase活性随土壤含盐量递增更呈直线性下降,对土壤盐分非常敏感。盐胁迫下,枣品种耐盐性不同,枣叶细胞中3种H+-酶活性的差异也很显著。总体上,耐盐性强的枣品种,质膜和液泡膜质子泵的活性均强,根尖细胞向质外体"排盐"的功能与向共质体的液泡中"固定盐分"的功能均突出,叶片细胞向质外体"排盐"的功能基本保持而向液泡中"固定盐分"的功能显著增强,但耐盐性弱的枣品种盐胁迫下叶片细胞质膜、液泡膜质子泵活性下降或略有增强。

Relationship between salt tolerances of Ziziphus jujube cv.Jinsixiaozao and other three cultivars and Proton Pump activities of plasma membrane and tonoplast in roots and leaves

The relationship between salt tolerances of Ziziphus jujube Mill.cv.Jinsixiaozao and other three cultivars mainly cultivated in East China and proton pump activities of plasma membrane and tonoplast in their roots and leaves were investigated under five levels of soil salt content by adding 0.0%,0.10%,0.30%,0.50% and 0.70% NaCl to culture medium.Analysis results about the relative water content,MDA content,relative eletrolytic leakage,Fv/Fo,Fv/Fm and net photosynthetic rate of leavese showed that salt toler...