| 摘 要： | The composition of fermentation medium for producing Cordyceps brasiliensis mycelium was optimized using response surface methodology. A Plackett-Burman design was applied to screen potential key factors selected on the basis of preliminary experiments, and the steepest ascent method was adopted to approach the approximate optimal medium composition. Finally, central composite design and response surface methodology were applied to define more precisely the optimal composition of the fermentation medium. Our data revealed glucose and beef extract to be the most influential parameters, and the composition of the optimal fermentation medium (100 mL) was determined to consist of 4.46 g glucose, 2 g soluble starch, 4 g silkworm powder, 2.93 g beef extract, 0.10 g KH2PO4 and 0.15 g MgSO4·7H2O.
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