| 高压均质法提取云芝胞内糖肽 |
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| 引用本文： | 陈露,陆震鸣,龚劲松,耿燕,徐国华,张晓梅,史劲松,许正宏.高压均质法提取云芝胞内糖肽[J].食用菌学报,2017(2):79-82. |
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| 作者姓名： | 陈露 陆震鸣 龚劲松 耿燕 徐国华 张晓梅 史劲松 许正宏 |
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| 作者单位： | 1. 江南大学药学院,江苏无锡,214122;2. 江苏神华药业有限公司,江苏淮安,211600 |
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| 摘 要： | 采用高压均质法提取云芝(Coriolus versicolor)胞内糖肽,与热水法进行比较。结果表明:高压均质提取的云芝胞内糖肽得率是热水提取的2倍,为(20.2±0.6)%;采用高压均质法提取的组分5相对百分含量最高,为48.07%;扫描电镜下观察发现,与热水提取相比,高压均质提取时云芝菌丝体破碎程度比较高。
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| 关 键 词： | 云芝 糖肽 高压均质 热水提取 高效液相色谱 |
Extraction of Intracellular Polysaccharopeptide from Coriolus versicolor Mycelium Using Hot Water and High-Pressure Homogenization |
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| CHEN Lu,LU Zhenming,GONG Jinsong,GENG Yan,XU Guohua,ZHANG Xiaomei,SHI Jinsong,XU Zhenghong.Extraction of Intracellular Polysaccharopeptide from Coriolus versicolor Mycelium Using Hot Water and High-Pressure Homogenization[J].Acta Edulis Fungi,2017(2):79-82. |
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| Authors: | CHEN Lu LU Zhenming GONG Jinsong GENG Yan XU Guohua ZHANG Xiaomei SHI Jinsong XU Zhenghong |
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| Abstract: | High-pressure homogenization (HPH) and hot water extraction (HWE)were used to extract intracellular polysaccharopeptide (IP) from Coriolus versicolor mycelia.Yields of IP extracted using HPH reached (20.2±0.6)%, which were twice as high as those achieved using HWE.HPLC separated the IPs into six components, of which the component with a peak retention time of 26.46 mins made up 48.07% of the material extracted using HPH.Scanning electron microscopy revealed that HPH-treated mycelium had a more porous and ruptured structure compared to HWE-treated samples. |
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| Keywords: | Coriolus versicolor polysaccharopeptide high-pressure homogenization hot water extraction high performance liquid chromatography |
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