| 香菇DNA导入平菇原生质体及转化子鉴定研究 |
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| 引用本文： | 贾建航,李小兵.香菇DNA导入平菇原生质体及转化子鉴定研究[J].食用菌学报,1997,4(4):5-10. |
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| 作者姓名： | 贾建航 李小兵 |
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| 作者单位： | [1]河北农业大学细胞工程育种课题组 [2]中国科学院遗传研究所 |
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| 基金项目： | 河北省自然科学基金资助 |
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| 摘 要： | 采用氯化苄方法提取香菇总ＤＮＡ，用Ｇｅｎｅ Ｐｕｌｓｅｒ Ⅱ电击系统将香菇ＤＮＡ导入平菇单核原生质体，通过香菇ＤＮＡ的导入，利用基因互补原理获得了能出菇的转化子１－１和１３－４８，并对转化子进行了拮抗试验、菌丝生长速度分析，酯酶同工酶分析和ＲＡＰＤ分析。
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| 关 键 词： | 香菇 平姑 电击 转化子 原生质体 |
Studies on the Introduction of Lentinula edodes DNA into Protoplasts of Pleurotus ostreatus and the Identification of Transformants |
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| Jia Jianhang Liu Zhenyue Tai Lifang Liu Guozhen Zhang Wei Li Liyun.Studies on the Introduction of Lentinula edodes DNA into Protoplasts of Pleurotus ostreatus and the Identification of Transformants[J].Acta Edulis Fungi,1997,4(4):5-10. |
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| Authors: | Jia Jianhang Liu Zhenyue Tai Lifang Liu Guozhen Zhang Wei Li Liyun |
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| Abstract: | Abstract The total DNA of Lentinula edodea isolated by the method of benzyl chloride was introduced into protoplasts of monokaryotic mycelia of P. oatreatua with Bio-Rad Gene Pulser n electroporation system. Optimal electroporation condition was selected in this study. Transfor-mants 1-1 and 13-48 with clamp connection were obtained and formed fruitbodies through principle of genie complementation after introducing L. edodea DNA. While the antagonism reaction test and the mycelium growth rate, esterase isozyme and RAPD analysis of the transformants were also carried out in this study. |
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| Keywords: | Lentinula edodes Pleurotus ostreatus Electroporation Transformant |
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