| 金针菇异戊烯基焦磷酸异构酶基因的克隆及表达分析 |
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| 引用本文： | 刘建雨,刘建辉,徐珍,于海龙,宋春艳,尚晓冬.金针菇异戊烯基焦磷酸异构酶基因的克隆及表达分析[J].食用菌学报,2016(3):10-14. |
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| 作者姓名： | 刘建雨 刘建辉 徐珍 于海龙 宋春艳 尚晓冬 |
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| 作者单位： | 上海市农业科学院食用菌研究所,农业部应用真菌资源与利用重点开放实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点实验室,上海201403 |
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| 基金项目： | 国家科技支撑项目(2013BAD16B02)，上海种业专项[沪农科种字(2012)第6号]资助 |
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| 摘 要： | PCR扩增了金针菇(Flammulina velutipes)异戊烯基焦磷酸异构酶基因(FvIDI)的DNA全长和开放阅读框序列.该基因的ORF全长753 bp,DNA全长923 bp,含有3个内含子和4个外显子,编码250个氨基酸,金针菇异戊烯基焦磷酸异构酶相对分子质量为28450,等电点为5.05.采用实时荧光定量PCR技术研究FvIDI基因在不同发育时期和不同温度下的表达量,结果显示,FvIDI基因在采收期菌盖中表达量最高,4℃诱导处理2～4 h和37℃诱导处理2～6 h的表达量明显高于常规温度21℃处理.
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| 关 键 词： | 金针菇 异戊烯基焦磷酸异构酶基因 实时荧光定量PCR技术 温度诱导 |
Cloning and Expression of an Isopentenyl Diphosphate Isomerase Gene from Flammulina velutipes |
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| Abstract: | The full-length DNA and open reading frame (ORF) sequences of an isopentenyl diphosphate isomerase (IDI) gene were cloned from Flammulina velutipes by PCR amplification.IDI was 923 bp in length,with an ORF of 753 bp encoding 250 amino acids,and contained three introns and four exons.IDI protein had a molecular mass of 28450 and a pI value of 5.57.Real-time quantitative PCR revealed that IDI expression levels were higher in mature pilei compared with the mycelial,primordium,stipe elongation,pileus elongation and stipe maturation stages.Expression levels were also higher in F.velutipes mycelia grown at 21 ℃ for 6 d before exposure to 4 ℃ for 2-4 h or to 37 ℃ for 2-6 h compared with controls maintained at 21 ℃. |
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| Keywords: | Flammulina velutipes isopentenyl diphosphate isomerase real-time PCR temperature-induced |
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