| 乌塌菜与薹菜变种间杂交后代硫代葡萄糖苷组分及含量分析 |
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| 引用本文： | 冯希环,孙艳,刘倩倩,汪俏梅,刘维信.乌塌菜与薹菜变种间杂交后代硫代葡萄糖苷组分及含量分析[J].中国蔬菜,2016,1(12):42. |
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| 作者姓名： | 冯希环 孙艳 刘倩倩 汪俏梅 刘维信 |
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| 作者单位： | 1.青岛农业大学园艺学院，青岛市园艺植物遗传改良与育种重点实验室，山东青岛 266109；;2. 浙江大学园;
艺系，农业部园艺植物生长发育与生物技术重点开放实验室，浙江杭州 310029 |
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| 基金项目： | 国家自然科学基金项目（31401864），山东省自然科学基金项目（ZR2016CM15），青岛市自主创新计划应用基础研究项目（16-5-1-74-jch） |
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| 摘 要： | 以乌塌菜品种上海小八叶、薹菜品种平度薹菜及变种间杂交后代6-1和6-5为试材,采用高效液相色谱法(HPLC)分析叶片及叶柄中硫代葡萄糖苷的组分与含量,以期了解不结球白菜变种间杂交后代的硫苷变化。结果表明:4份材料的叶片、叶柄中均检测到组分相同的脂肪族硫苷、吲哚族硫苷各4种,而芳香族硫苷只在变种间杂交后代6-1的叶片和叶柄中检测到;参试各材料叶片中总脂肪族硫苷含量、总吲哚族硫苷含量和总硫苷含量均高于叶柄。变种间杂交后代6-1叶片、叶柄中有益人体健康的2-苯乙基硫苷(NAS)和吲哚-3-甲基硫苷(GBC)含量均高于亲本上海小八叶和平度薹菜,表明变种间杂交可以用于改良不结球白菜硫苷组分及含量。
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| 关 键 词： | 乌塌菜 薹菜 变种间杂交 硫代葡萄糖苷 高效液相色谱法 |
Analysis of Glucosinolates Composition and Content in Filial Lines Derived from Inter-Varietal Cross of Savoy and Tai-tsai by HPLC |
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| FENG Xi-huan,SUN Yan,LIU Qian-qian,WANG Qiao-mei,LIU Wei-xin.Analysis of Glucosinolates Composition and Content in Filial Lines Derived from Inter-Varietal Cross of Savoy and Tai-tsai by HPLC[J].China Vegetables,2016,1(12):42. |
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| Authors: | FENG Xi-huan SUN Yan LIU Qian-qian WANG Qiao-mei LIU Wei-xin |
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| Institution: | 1. College of Horticulture，Qingdao Agricultural University，Qingdao Key Laboratory of Genetic Improvement and Breeding;
in Horticultural Plants，Qingdao 266109，Shandong，China；;2. Department of Horticulture，Zhejiang University，;
Key Laboratory of Horticultural Plant Growth，Development and Quality Improvement，Ministry of Agriculture，Hangzhou;
310029，Zhejiang，China |
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| Abstract: | To understand the variation of glucosinolates composition in backcrossing lines derived
from inter-varietal crosses in pakchoi，the content and composition of glucosinolates were analyzed by high
performance liquid chromatography（HPLC）for savoy〔Brassica campestris L. ssp. chinensis（L.）Makino
var. rosularis Tsen et Lee〕cv.‘Shanghaixiaobaye’，tai-tsai〔Brassica campestris L. ssp. chinensis（L.）
Makino var. tai-tsai Hort〕cv.‘Pingdutaicai’，and 2 lines selected from F2 populations of 4 times backcrossing of
（‘Shanghaixiaobaye’בPingdutaicai’）with‘Shanghaixiaobaye’as the recurrent parent．The results
showed that each of 4 kinds of aliphatic and indole glucosinolate were detected in the leaf and petiole of 4 tested
materials．The total contents of aliphatic glucosinolate，indole glucosinolate and total glucosinolate in leaves were
higher than petioles，and also different with 4 tested materials．The contents of health-beneficial compounds
2-phenylethyl glucosinolate（NAS）and glucobrassicin（GBC）were higher in leaves of‘6-1’than both its
parents‘Shanghaixiaobaye’and‘Pingdutaicai’，indicating that inter-varietal crosses could be used in breeding
new pakchoi cultivars with improved glucosinolate composition． |
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| Keywords: | Savoy Tai-tsai Inter-varietal cross Glucosinolate HPLC |
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